Strain Data Sheet

RBRC11067

Information1

Image
BRC No.RBRC11067
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;B6CB-Fgf10<tm1Ska>
Former Common nameFgf10<tm1Ska>, Fgf10 knockout mouse
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeposited by Hideyo Ohuchi, Okayama University in 2020. Mice were generated using TT2 (ES Cell) derived from (C57BL/6NCrlj x CBA/JNCrlj)F1. Mixed genetic background.
Strain descriptionFgf10 knockout mouse. A neomycin resistance cassette replaced 1.1 kb of sequence that contains the exon encoding the ATG translation initiation site and the amino terminal 110 amino acids of the encoded protein.
Colony maintenance
ReferencesNat. Genet., 21(1):138-41 (1999). 9916808

Health Report

Examination Date / Room / Rack2021/05/17Room:3-CRack:L
2021/02/15Room:3-CRack:L
2021/01/25Room:3-CRack:L
2020/11/16Room:3-CRack:L

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Fgf10fibroblast growth factor 1013Fgf10<tm1Ska>targeted mutation 1, Shigeaki Kato

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)13mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 PolyA signal13

Information2

Donor DNAmouse Phosphoglycerate kinase promoter, E. coli Neomycin resistant gene, SV40 PolyA signal, mouse Fgf10 genomic DNA
Research application
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat. Genet., 21(1):138-41 (1999).For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorHideyo Ohuchi (Okayama University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data