Strain Data Sheet

RBRC11067

Strain Information

Image
BRC No.RBRC11067
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;B6CB-Fgf10<tm1Ska>
Former Common nameFgf10<tm1Ska>, Fgf10 knockout mouse
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeposited by Hideyo Ohuchi, Okayama University in 2020. Mice were generated using TT2 (ES Cell) derived from (C57BL/6NCrlj x CBA/JNCrlj)F1. Mixed genetic background.
Strain descriptionFgf10 knockout mouse. A neomycin resistance cassette replaced 1.1 kb of sequence that contains the exon encoding the ATG translation initiation site and the amino terminal 110 amino acids of the encoded protein.
Colony maintenance
References
Fgf10 is essential for limb and lung formation.
Sekine K, Ohuchi H, Fujiwara M, Yamasaki M, Yoshizawa T, Sato T, Yagishita N, Matsui D, Koga Y, Itoh N, Kato S
Nat. Genet., 21(1):138-41 (1999). 9916808

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
SV40 polyA signal13
  • aplasia of lacrimal and salivary glands(MedGEN)

  • LADD syndrome(DisGeNET, MedGEN)
    • Fgf10fibroblast growth factor 1013Fgf10targeted mutation 1, Shigeaki Kato
  • aplasia of lacrimal and salivary glands(MedGEN)

  • LADD syndrome(DisGeNET, MedGEN)
    • neoneomycin resistance gene (E. coli)13mouse phosphoglycerate kinase promoter (PGK promoter)
  • aplasia of lacrimal and salivary glands(MedGEN)

  • LADD syndrome(DisGeNET, MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
    Detailed phenotype data

    Ordering Information

    Donor DNAmouse Phosphoglycerate kinase promoter, E. coli Neomycin resistant gene, SV40 PolyA signal, mouse Fgf10 genomic DNA
    Research application
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat. Genet., 21(1):138-41 (1999).For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorHideyo Ohuchi (Okayama University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data