Strain Data Sheet

RBRC11067

Strain Information

Image
BRC No.RBRC11067
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;B6CB-Fgf10<tm1Ska>
Former Common nameFgf10<tm1Ska>, Fgf10 knockout mouse
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeposited by Hideyo Ohuchi, Okayama University in 2020. Mice were generated using TT2 (ES Cell) derived from (C57BL/6NCrlj x CBA/JNCrlj)F1. Mixed genetic background.
Strain descriptionFgf10 knockout mouse. A neomycin resistance cassette replaced 1.1 kb of sequence that contains the exon encoding the ATG translation initiation site and the amino terminal 110 amino acids of the encoded protein.
Colony maintenance
References
Fgf10 is essential for limb and lung formation.
K Sekine, H Ohuchi, M Fujiwara, M Yamasaki, T Yoshizawa, T Sato, N Yagishita, D Matsui, Y Koga, N Itoh, S Kato
Nat. Genet., 21(1):138-41 (1999). 9916808

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Fgf10
MGI:1099809		fibroblast growth factor 1013Fgf10<tm1Ska> targeted mutation 1, Shigeaki Kato
  • aplasia of lacrimal and salivary glands(MedGEN)

  • LADD syndrome(DisGeNET, MedGEN)
    • neo neomycin resistance gene (E. coli)13 mouse phosphoglycerate kinase promoter (PGK promoter)
    SV40 polyA signal13

    Phenotype

    Phenotype annotation from literatures by Mammalian phenotype ontology
  • abnormal Rathke's pouch apoptosis (MP:0013352)

  • abnormal adipose tissue morphology (MP:0000003)

  • abnormal branching of the mammary ductal tree (MP:0000662)

  • abnormal cecum development (MP:0009479)

  • abnormal colon morphology (MP:0000495)
    • more 78 phenotypes
  • abnormal embryonic tissue morphology (MP:0002085)

  • abnormal enzyme/coenzyme activity (MP:0005584)

  • abnormal forced expiratory flow rates (MP:0002297)

  • abnormal gastric chief cell morphology (MP:0004140)

  • abnormal hair follicle morphology (MP:0000377)

  • abnormal hair shaft morphology (MP:0003809)

  • abnormal heart atrium auricular region morphology (MP:0010489)

  • abnormal heart position or orientation (MP:0006065)

  • abnormal hindgut morphology (MP:0008786)

  • abnormal incisor morphology (MP:0005358)

  • abnormal inner ear morphology (MP:0000026)

  • abnormal intestinal epithelium morphology (MP:0000488)

  • abnormal kidney inner medulla morphology (MP:0011333)

  • abnormal kidney medulla morphology (MP:0003014)

  • abnormal kidney outer medulla morphology (MP:0011332)

  • abnormal large intestine crypts of Lieberkuhn morphology (MP:0004842)

  • abnormal limb bud morphology (MP:0005650)

  • abnormal limb development (MP:0006279)

  • abnormal limb muscle morphology (MP:0021206)

  • abnormal lung development (MP:0001176)

  • abnormal mammary fat pad morphology (MP:0008904)

  • abnormal mammary placode morphology (MP:0013721)

  • abnormal metanephric ureteric bud development (MP:0011457)

  • abnormal neurohypophysis development (MP:0013338)

  • abnormal periderm development (MP:0013531)

  • abnormal pulmonary circulation (MP:0002295)

  • abnormal pulmonary elastic fiber morphology (MP:0003209)

  • abnormal pulmonary trunk morphology (MP:0000486)

  • abnormal scapula morphology (MP:0000149)

  • abnormal stomach submucosa morphology (MP:0010794)

  • abnormal submandibular duct morphology (MP:0009525)

  • abnormal tooth morphology (MP:0002100)

  • abnormal urethra morphology (MP:0000537)

  • absent Rathke's pouch (MP:0011759)

  • absent adenohypophysis (MP:0008320)

  • absent colon (MP:0011884)

  • absent forelimb (MP:0000551)

  • absent hindlimb (MP:0000557)

  • absent humerus (MP:0004352)

  • absent limbs (MP:0000549)

  • absent lung buds (MP:0010907)

  • absent lungs (MP:0001181)

  • absent pituitary gland (MP:0005315)

  • absent pulmonary artery (MP:0010521)

  • absent pulmonary vein (MP:0010646)

  • absent radius (MP:0000553)

  • absent rectum (MP:0009509)

  • absent salivary gland (MP:0000614)

  • absent submandibular gland (MP:0009524)

  • absent thyroid gland (MP:0005314)

  • absent ulna (MP:0004360)

  • cecal atresia (MP:0009510)

  • decreased body weight (MP:0001262)

  • decreased colon length (MP:0013956)

  • decreased hair follicle number (MP:0000379)

  • decreased pancreatic beta cell number (MP:0003339)

  • decreased trachea gland number (MP:0013495)

  • dextrocardia (MP:0000644)

  • diaphragmatic hernia (MP:0003924)

  • dilated kidney calyx (MP:0011305)

  • eyelids open at birth (MP:0001302)

  • impaired branching involved in bronchus morphogenesis (MP:0011028)

  • increased hair follicle apoptosis (MP:0011195)

  • pancreas fibrosis (MP:0003334)

  • perinatal lethality, complete penetrance (MP:0011089)

  • perineal hypospadia (MP:0031300)

  • short incisors (MP:0004830)

  • small hair follicle bulb (MP:0030920)

  • small kidney (MP:0002989)

  • small lung (MP:0003641)

  • small molars (MP:0006402)

  • small otic capsule (MP:0006288)

  • small pancreas (MP:0004247)

  • small stomach (MP:0002691)

  • small thymus (MP:0000706)

  • submandibular gland hypoplasia (MP:0009522)

  • thin skin (MP:0001199)

  • translucent skin (MP:0001201)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse Phosphoglycerate kinase promoter, E. coli Neomycin resistant gene, SV40 PolyA signal, mouse Fgf10 genomic DNA
    Research applicationMouse Models for Human Disease
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat. Genet., 21(1):138-41 (1999).For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorHideyo Ohuchi (Okayama University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data