Strain Data Sheet

RBRC11066

Strain Information

Image
BRC No.RBRC11066
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameC57BL/6N-Del(16Pi4ka-Hira)Atai
Former Common nameDel(3.0Mb)/+
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Atsu Aiba, The University of Tokyo in 2020. Mice were generated using C57BL/6N with the CRISPR/Cas9. C57BL/6N genetic background.
Strain descriptionDeletion mutant which lacks genomic region of Pi4ka-Hira in chromosome 16. Mouse model for human 22q11.2 deletion syndrome.
Colony maintenance
References
Comprehensive analysis of a novel mouse model of the 22q11.2 deletion syndrome: a model with the most common 3.0-Mb deletion at the human 22q11.2 locus.
Saito R, Koebis M, Nagai T, Shimizu K, Liao J, Wulaer B, Sugaya Y, Nagahama K, Uesaka N, Kushima I, Mori D, Maruyama K, Nakao K, Kurihara H, Yamada K, Kano M, Fukada Y, Ozaki N, Aiba A
Transl. Psychiatry, 10(1):35 (2020). 32066675

Health Report

Examination Date / Room / Rack2024/10/21Room:3-CRack:B
2024/04/22Room:3-CRack:B
2024/01/22Room:3-CRack:B
2023/10/23Room:3-CRack:B
2023/07/24Room:3-CRack:B
2023/05/22Room:3-CRack:B
2023/04/24Room:3-CRack:B
2023/01/23Room:3-CRack:B

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Hirahistone cell cycle regulator16Hira
  • polymicrogyria, perisylvian, with cerebellar hypoplasia and arthrogryposis(MedGEN)
    • Pi4kaphosphatidylinositol 4-kinase alpha16Pi4ka
  • polymicrogyria, perisylvian, with cerebellar hypoplasia and arthrogryposis(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
    Detailed phenotype data

    Ordering Information

    Donor DNAStreptococcus pyogenes SpCas9 (human codon-optimized)*, Synthetic sgRNA, Synthetic ssODN* Not detected by PCR using Marker Gene Detection kit (TOYOBO, Osaka, Japan). Other introduced genes were not tested.
    Research applicationMouse Models for Human Disease
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Transl. Psychiatry, 10(1):35 (2020).
    DepositorAtsu Aiba (The University of Tokyo)
    Strain Statusan icon for Live miceLive mice
    an icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityCryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-
    Mouse of the Month Nov 2021

    BRC mice in Publications

    No Data