Strain Data Sheet

RBRC11066

Information1

Image
BRC No.RBRC11066
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameC57BL/6N-Del(16Pi4ka-Hira)Atai
Former Common nameDel(3.0Mb)/+
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Atsu Aiba, The University of Tokyo in 2020. Mice were generated using C57BL/6N with the CRISPR/Cas9. C57BL/6N genetic background.
Strain descriptionDeletion mutant which lacks genomic region of Pi4ka-Hira in chromosome 16. Mouse model for human 22q11.2 deletion syndrome.
Colony maintenance
ReferencesTransl. Psychiatry, 10(1):35 (2020). 32066675

Health Report

Examination Date / Room / Rack2022/07/25Room:3-CRack:B
2022/04/25Room:3-CRack:B
2022/02/14Room:3-CRack:B
2021/11/15Room:3-CRack:B
2021/11/01Room:3-CRack:B
2021/10/24Room:3-CRack:B
2021/08/16Room:3-CRack:B
2021/05/17Room:3-CRack:B

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Pi4kaphosphatidylinositol 4-kinase alpha16Pi4ka

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Hirahistone cell cycle regulator16Hira

Information2

Donor DNAStreptococcus pyogenes SpCas9 (human codon-optimized)*, Synthetic sgRNA, Synthetic ssODN* Not detected by PCR using Marker Gene Detection kit (TOYOBO, Osaka, Japan). Other introduced genes were not tested.
Research applicationMouse Models for Human Disease
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Transl. Psychiatry, 10(1):35 (2020).
DepositorAtsu Aiba (The University of Tokyo)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved embryos (within 1 month)
Live mouse (1 to 3 months)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data