Strain Data Sheet

RBRC11036

Information1

Image
BRC No.RBRC11036
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameC57BL/6N.Cg-Adam28<em2Osb>
Former Common nameAdam28<-47616/wt>
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2020. Mice were generated using EGR-G01 (ES cell) derived from (129S2 x B6Cr)F1-Tg(CAG/Acr-EGFP). Mice were further crossed to C57BL/6N. C57BL/6N congenic line.
Strain descriptionAdam28 knockout mouse generated by the CRISPR/Cas9. 47616 bp deletion in the Adam28 gene. Homozygous mice tend to exhibit decreased fertility.
Colony maintenance
ReferencesScience, 368(6495):1132-1135 (2020). 32499443

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Adam28a disintegrin and metallopeptidase domain 2814Adam28<em2Osb>endonuclease-mediated mutation 2, Research Institute for Microbial Diseases, Osaka University

Information2

Donor DNAhuman hU6 promoter, CMV enhancer (CBh), chicken chicken beta-actin promoter (CBh), SV40 nuclear localization signal, Streptococcus pyogenes SpCas9 (human codon-optimized), crRNA, tracrRNA, Escherichia coli ampicillin resistant gene, Streptomyces alboniger puromycin resistant gene, Mouse a part of Adam28 gene
Research application
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Science, 368(6495):1132-1135 (2020).The RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorMasahito Ikawa (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP

BRC mice in Publications

No Data