Strain Data Sheet

RBRC11014

Strain Information

Image
BRC No.RBRC11014
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameB6D2-Tmprss12<em4Osb>
Former Common nameTmprss12<-8,-7871+5/wt>
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain development
Strain descriptionTmprss12 KO mouse generated by the CRISPR/Cas9. 7871 bp and 8 bp deletion in the Tmprss12 gene. Homozygous male mice are infertile.
Colony maintenance
References
Tmprss12 is required for sperm motility and uterotubal junction migration in micedagger.
Larasati T, Noda T, Fujihara Y, Shimada K, Tobita T, Yu Z, Matzuk M M, Ikawa M
Biol. Reprod., 103(2):254-263 (2020). doi: 10.1093/biolre/ioaa060. 32529245

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Tmprss12transmembrane (C-terminal) protease, serine 1215Tmprss12endonuclease-mediated mutation 4, Research Institute for Microbial Diseases, Osaka University

Phenotype

Annotation by Mammalian phenotyhpe ontology
Detailed phenotype data

Ordering Information

Donor DNAStreptococcus pyogenes crRNA, tracrRNA, Mouse a part of Tmprss12 gene
Research application
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biol. Reprod., 103(2):254-263 (2020). doi: 10.1093/biolre/ioaa060.The RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorMasahito Ikawa (Osaka University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

No Data