Strain Data Sheet

RBRC10994

Strain Information

Image
BRC No.RBRC10994
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6-Tardbp<tm1Koya>
Former Common nameTDP-DeltaC, TDP-dC
H-2 Haplotype
ES Cell lineRENKA [C57BL/6NCrlCrlj]
Background strain
Appearance
Strain developmentDeveloped and deposited by Dr. Koji Yamanaka. Miice were generated using RENKA ES cells (C57BL/6 N). C57BL/6 genetic background.
Strain descriptionKockin mice in which a part of exon 6, encoding amino acid 274-414, was replaced with a 3FLAG tag to delete the C-terminal portion of the encoded protein.
Colony maintenance
References
Mice deficient in the C-terminal domain of TAR DNA-binding protein 43 develop age-dependent motor dysfunction associated with impaired Notch1-Akt signaling pathway.
Kohei Nishino, Seiji Watanabe, Jin Shijie, Yuri Murata, Kotaro Oiwa, Okiru Komine, Fumito Endo, Hitomi Tsuiji, Manabu Abe, Kenji Sakimura, Amit Mishra, Koji Yamanaka
Acta Neuropathol. Commun., 7(1):118 (2019). 31345270

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
FLAG 3xFLAG tag (synthetic)4FLAG
Frt yeast FRT (flippase recombination target) site4Frt
Tardbp
MGI:2387629		TAR DNA binding protein4Tardbp<tm1Koya> targeted mutation 1, Koji Yamanaka
  • amyotrophic lateral sclerosis type 10(DisGeNET, MedGEN)
    • neo neomycin resistance gene (E. coli)4 mouse phosphoglycerate kinase promoter (PGK promoter)

    Phenotype

    Phenotype annotation from literatures by Mammalian phenotype ontology
  • abnormal cholinergic neuron morphology (MP:0001038)

  • abnormal motor capabilities/coordination/movement (MP:0002066)

  • abnormal motor neuron morphology (MP:0000937)

  • embryonic lethality, complete penetrance (MP:0011092)
    • Detailed phenotype data

    Ordering Information

    Donor DNAphage T7 gb2 promoter, ネオマイシン耐性遺伝子, マウス Phosphoglycerate kinase I(PGK-1)プロモーターおよびポリA配列, 酵母 Saccharomyces cerevisiae FRT配列, 合成DNA FLAG タグ
    Research applicationFLP/frt system
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Acta Neuropathol Commun., 7(1):118 (2019). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
    DepositorKoji Yamanaka (RIKEN)
    Strain Statusan icon for Frozen embryosFrozen embryos
    Strain AvailabilityRecovery and QC required prior to distribution
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    BRC mice in Publications

    No Data