RBRC10984

Information1

Image
BRC No.RBRC10984
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6;B6D2-Tg(CAG-hCas9)2Saku
Former Common namesCAT (systemically Cas9-expressing Transgenic mice)
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDevekoped by Takayuki Sakurai, Shinshu University in 2013. A TG construct was injected into eggs derived from B6D2FlxB6J. Mixed genetic background.
Strain descriptionTransgenic mice expressing hCas9 under the control of CAG promoter.
Colony maintenance
ReferencesSci. Rep., 10(1):1091 (2020). 31974430
Sci. Rep., 6:20011 (2016). 26817415

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Cas9CRISPR associated protein 9UNCas9CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
FLAGFLAG tag (synthetic)UNFLAG

Information2

Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Synthetic Flag tag, Streptococcus pyogenes Cas9 cDNA
Research application
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Sci Rep. 2016 Jan 28;6:20011, Sci Rep. 2020; 10: 1091.In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE b a for-profit institution the RECIPIENT must reach a agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
DepositorTakayuki Sakurai (Shinshu University)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. CAGGS MTA (English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data