Strain Data Sheet

RBRC10983

Information1

Image
BRC No.RBRC10983
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Hcn4<tm1(tTA)Nnka> Hcn4<tm1.1(Luc/EGFP)Nna>
Former Common nameHCN4 tet/luc mice
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Noriyuki Nakashima and Makoto Takano, Kurume University in 2017.
Strain descriptionThis strain has two different alleles at the Hcn4 gene locus. One is a reporter allele in which luciferase/EGFP fusion gene was inserted in frame of translation initiation codon of the Hcn4 gene. Hcn4 gene is disrupted. The other is an allele in which tTA/Tre cassette was inserted at the translation initiation site of the Hcn4 gene.
Colony maintenance
ReferencesJ. Physiol., 596(5):809-825 (2018). 29315578

Health Report

Examination Date / Room / Rack2022/06/13Room:3-3Rack:D
2022/03/14Room:3-3Rack:D
2021/12/14Room:3-3Rack:D
2021/09/13Room:3-3Rack:D
2021/06/14Room:3-3Rack:D
2021/03/29Room:3-3Rack:D
2021/01/05Room:3-3Rack:D

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Hcn4hyperpolarization-activated, cyclic nucleotide-gated K+ 49Hcn4<tm1.1(Luc/EGFP)Nna>targeted mutation 1.1, Noriyuki Nakashima

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Hcn4hyperpolarization-activated, cyclic nucleotide-gated K+ 49Hcn4<tm1(tTA)Nnka>targeted mutation 1, Noriyuki Nakashima

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)9EGFP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GHhuman growth hormone gene9GH

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
URA3Orotidine-5'-phosphate (OMP) decarboxylase (yeast)9URA3

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP9loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP9loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP9loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lucluciferase9luc

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)9herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
tTAtTA (E. coli tet repressor (TetR), herpes simplex virus (HSV) VP16 activation domain)9tTAE. coli tet repressor (TetR), herpes simplex virus (HSV)VP16 activation domain

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 PolyA signal9

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 PolyA signal9

Information2

Donor DNAfirefly Luciferase cDNA, jellyfish EGFP cDNA, Simian virus SV40, P1 phage loxP, Human human growth hormone gene, cytomegalovirus cytomegalovirus promoter, herpes simplex virus HSV thymidine kinase polyadenylation signal、HSV thymidine kinase promoter, E coli tetracycline transactivator、tet operator、neomycin resistant gene、yeast URA3 gene
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Tet system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Physiol., 596(5):809-825 (2018).For use of the BIOLOGICAL RESOURCE by a for-profit institution the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
DepositorMakoto Takano (Kurume University)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Live mouse (1 to 3 months)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)
  4. GFP Transfer License (Japanese / English)
    Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

Genotyping protocol -PCR-

BRC mice in Publications

No Data