Strain Data Sheet

RBRC10980

Information1

Image
BRC No.RBRC10980
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6NJcl.Cg-Mdga2<tm1Tymo>
Former Common nameMdga2 KO
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Tohru Yamamoto, Faculty of Pharmaceutical Science, Hokkaido University. TT2 (B6CBF1 derived) ES cells were used to generate Mdga2 knockout mice. The mutant mice were backcrossed to C57BL/6N. C57BL/N congenic strain.
Strain descriptionMdga2 gene knockout mice. Exon 1 of the Mdga2 gene was replaced with lacZ and neo cassette.
Colony maintenance
ReferencesNeuron. 2016 Sep 7;91(5):1052-1068 27608760
PLoS Genet. 2009 Jun;5(6):e1000536. 19557195

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Mdga2MAM domain containing glycosylphosphatidylinositol anchor 212Mdga2<tm1Tymo>targeted mutation 1, Toru Yamamoto

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site12Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site12Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)12

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lox71mutant lox sequence12lox71

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP12loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)12mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAP1 phage lox71 site, E.coli LacZ, rabbit beta-globin 3’end, Yeast frt sites, mouse PGK promoter, E.coli neo, P1 phage loxP site, mouse PGK 3’end, mouse Mdga2 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Acc.No. CDB0704K and Web. Site (http://www2.clst.riken.jp/arg/mutant%20mice%20list.html) is requested. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested. (Neuron. 91(5):1052-68.) Please inform theDEPOSITOR (mutant.bdr@riken.jp) about the paper when published.
DepositorTohru Yamamoto (Kagawa University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data