Strain Data Sheet

RBRC10978

Information1

Image
BRC No.RBRC10978
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6NJcl.Cg-Mdga1<tm1.1Tymo>
Former Common nameMdga1 KO
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Tohru Yamamoto, Faculty of Pharmaceutical Science, Hokkaido University. TT2 (B6CBF1 derived) ES cells were used to generate Mdga1 knockout mice. The mutant mice were backcrossed to C57BL/6N. C57BL/N congenic strain.
Strain descriptionMdga1 gene knockout mice. Exon 1 of the Mdga1 gene was replaced with a lacZ and frt flanked neo cassette (neo cassette was removed by FLPe-mediated recombination). Homozygous mutant mice exhibit defective migration and positioning of neurons during corticogenesis.
Colony maintenance
ReferencesSchizophr Res. 2011 Feb;125(2-3):194-200 21146959
Am. J. Med. Genet. B Neuropsychiatr. Genet., 2008 Oct 5;147B(7):1089-100. 18384059
Brain Res. 2006 Jul 26;1101(1):12-9. Epub 2006 Jun 19. 16782075
Neurosci Lett., 716:134677 (2020). 31812551
Dev. Dyn., 240(1):96-107 (2011). 21104742
Cell Rep. 2017 Dec 26;21(13):3637-3645 29281813

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Mdga1MAM domain containing glycosylphosphatidylinositol anchor 117Mdga1<tm1.1Tymo>targeted mutation 1.1, Toru Yamamoto

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site17Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)17

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lox71mutant lox sequence17lox71

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP17loxP

Information2

Donor DNAP1 phage lox71 site, E.coli LacZ, rabbit beta-globin 3’end, Yeast frt site, P1 phage loxP site, mouse PGK 3’end, mouse Mdga1 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Acc.No. CDB0454K and Web. Site (http://www2.clst.riken.jp/arg/mutant%20mice%20list.html) is requested. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested. (Dev Dyn. 2011 240(1):96-107.) Please inform the DEPOSITOR (mutant.bdr@riken.jp) about the paper when published.
DepositorTohru Yamamoto (Kagawa University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data