Strain Data Sheet
RBRC10972
Information1 | |
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| Image | |
| BRC No. | RBRC10972 |
| Type | Targeted Mutation Congenic |
| Species | Mus musculus |
| Strain name | B6.Cg-Serpinh1<tm2Kzn> |
| Former Common name | Hsp47 flox |
| H-2 Haplotype | |
| ES Cell line | KY1.1[(C57BL/6J x 129S6/SvEvTac)F1] |
| Background strain | |
| Appearance | |
| Strain development | Developed by Kazuhiro Nagata, Kyoto University in 2012. KY1.1 ES cells derived from (C57BL/6J x 129S6/SvEvTac)F1 were used. C57BL/6 congenic strain by backcrossing for 7 generations. |
| Strain description | Serpinh1 (Hsp47) floxed mice. A loxP site was inserted upstream of exon 6. An FRT-flanked neo cassette with a 3'loxP site was inserted downstream of exon 6. |
| Colony maintenance | |
| References | J. Cell Sci., 125(Pt 5):1118-28 (2012). 22492985 |
Health Report | |
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| Examination Date / Room / Rack | 2022/07/11Room:3-ARack:L 2022/04/11Room:3-ARack:L 2022/01/24Room:3-ARack:L 2021/12/16Room:3-ARack:L |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Serpinh1serine (or cysteine) peptidase inhibitor, clade H, member 17Serpinh1<tm2Kzn>targeted mutation 2, Kazuhiro Nagata Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site7Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site7Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP7loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP7loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)7mouse Phosphoglycerate kinase promoter (PGK promoter), E. coli EM7 promoter |
Information2 | |
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| Donor DNA | bacteriophage P1 loxP sites, yeast FRT (flippase recombination target) sites, Mouse Phosphoglycerate kinase promoter(PGK promoter), E. coli EM7 promoter, E. coli Neomycin resistant gene, mouse Serpinh1 genomic DNA |
| Research application | Cre/loxP system FLP/frt system |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Cell Sci., 125(Pt 5):1118-28 (2012).The RECIPIENT of BIOLOGICAL RESOURCE must use it as a collaborative study with DEPOSITOR. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. |
| Depositor | Kazuhiro Nagata (Kyoto Sangyo University) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |