Strain Data Sheet

RBRC10972

Information1

Image
BRC No.RBRC10972
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Serpinh1<tm2Kzn>
Former Common nameHsp47 flox
H-2 Haplotype
ES Cell lineKY1.1[(C57BL/6J x 129S6/SvEvTac)F1]
Background strain
Appearance
Strain developmentDeveloped by Kazuhiro Nagata, Kyoto University in 2012. KY1.1 ES cells derived from (C57BL/6J x 129S6/SvEvTac)F1 were used. C57BL/6 congenic strain by backcrossing for 7 generations.
Strain descriptionSerpinh1 (Hsp47) floxed mice. A loxP site was inserted upstream of exon 6. An FRT-flanked neo cassette with a 3'loxP site was inserted downstream of exon 6.
Colony maintenance
ReferencesJ. Cell Sci., 125(Pt 5):1118-28 (2012). 22492985

Health Report

Examination Date / Room / Rack2022/07/11Room:3-ARack:L
2022/04/11Room:3-ARack:L
2022/01/24Room:3-ARack:L
2021/12/16Room:3-ARack:L
2021/10/26Room:3-ARack:L
2021/07/26Room:3-ARack:L
2021/04/26Room:3-ARack:L
2021/02/15Room:3-ARack:L
2021/02/01Room:3-ARack:L
2020/11/09Room:3-ARack:L

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Serpinh1serine (or cysteine) peptidase inhibitor, clade H, member 17Serpinh1<tm2Kzn>targeted mutation 2, Kazuhiro Nagata

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site7Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site7Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP7loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP7loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)7mouse Phosphoglycerate kinase promoter (PGK promoter), E. coli EM7 promoter

Information2

Donor DNAbacteriophage P1 loxP sites, yeast FRT (flippase recombination target) sites, Mouse Phosphoglycerate kinase promoter(PGK promoter), E. coli EM7 promoter, E. coli Neomycin resistant gene, mouse Serpinh1 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Cell Sci., 125(Pt 5):1118-28 (2012).The RECIPIENT of BIOLOGICAL RESOURCE must use it as a collaborative study with DEPOSITOR. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
DepositorKazuhiro Nagata (Kyoto Sangyo University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data