Strain Data Sheet

RBRC10970

Strain Information

Image
BRC No.RBRC10970
TypeCRISPR/Cas9 (Transgene)Cartagena
SpeciesMus musculus
Strain nameB6(Cg)-Sox17<em1(phiC31<*>)Okn>
Former Common nameTriple lineagelabelling mice (Sox17 2A-phiC31o Delta Puro)
H-2 Haplotype
ES Cell lineB6NJ-22<UTR> [C57BL/6NCrlCrlj]
Background strain
Appearance
Strain developmentDeposited by Hideyuki Okano, Keio University in 2020. C57BL/6 genetic background.
Strain descriptionKnock-in mice of phiC31o at the C-terminus of protein coding sequence of the Sox17 gene.
Colony maintenance
ReferencesDevelopment, 146(21). pii: dev174938 (2019). 31597657

Health Report

Examination Date / Room / Rack2023/08/28Room:4-BRack:D
2023/05/29Room:4-BRack:D
2023/04/10Room:4-BRack:D
2023/02/27Room:4-BRack:D
2022/11/29Room:4-BRack:D
2022/08/29Room:4-BRack:D
2022/05/31Room:4-BRack:D
2022/03/07Room:4-BRack:D

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Sox17SRY (sex determining region Y)-box 171Sox17<em1(phiC31<*>)Okn>endonuclease-induced mutation 1, Hideyuki Okano

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
F5yeast FRT (flippase recombination target) site (synthetic) variant1F5

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
P2Aporcine teschovirus-1 2A peptide1P2A

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
PhiC31oPhiC31 recombinase1PhiC31orat tyrosine hydroxylase (TH) promoter

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
nlsSV40 large T antigen nuclear localization signal (NLS)1nls

Ordering Information

Donor DNAP2A (porcine teschovirus-1 2A peptide), SV40 virus NLS, Streptomyces lividans phiC31o, Saccharomyces cerevisia FRT site(F5), mouse Sox17 genomic DNA
Research application
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Development. 2019 Nov 4;146(21). pii: dev174938
DepositorHideyuki Okano (Keio University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

No Data