Strain Data Sheet

RBRC10969

Strain Information

Image
BRC No.RBRC10969
TypeCRISPR/Cas9 (Transgene)Cartagena
SpeciesMus musculus
Strain nameB6(Cg)-T<em1(dre)Okn>
Former Common nameTriple lineagelabelling mice (brachyury2A-Dre Delta Puro)
H-2 Haplotype
ES Cell lineB6NJ-22<UTR> [C57BL/6NCrlCrlj]
Background strain
Appearance
Strain developmentDeposited by Hideyuki Okano, Keio University in 2020. C57BL/6 genetic background.
Strain descriptionKnock-in mice of Dre at the C-terminus of protein coding sequence of the T gene.
Colony maintenance
ReferencesDevelopment, 146(21). pii: dev174938 (2019). 31597657

Health Report

Examination Date / Room / Rack2023/11/27Room:4-BRack:H
2023/08/30Room:4-BRack:H
2023/08/28Room:4-BRack:H
2023/05/29Room:4-BRack:H
2023/02/27Room:4-BRack:H
2023/02/20Room:4-BRack:H
2022/11/29Room:4-BRack:H
2022/08/29Room:4-BRack:H
2022/05/31Room:4-BRack:H
2022/03/07Room:4-BRack:H

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Tbrachyury17T<em1(dre)Okn>endonuclease-induced mutation 1, Hideyuki Okano

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
DreBacteriophage D6 recombinase17Dre

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
F3yeast FRT (flippase recombination target) site (synthetic) variant17F3

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
P2Aporcine teschovirus-1 2A peptide17P2A

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
nlsSV40 large T antigen nuclear localization signal (NLS)17nls

Ordering Information

Donor DNAmouse T/brachyury genomic DNA, P2A (porcine teschovirus-1 2A peptide), P1-like transducing phage D6 Dre, SV40 virus NLS, Saccharomyces cerevisia FRT variant(F3)
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Development. 2019 Nov 4;146(21). pii: dev174938
DepositorHideyuki Okano (Keio University)
Strain Statusan icon for Live miceLive mice
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Live mouse (1 to 3 months)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

No Data