Strain Data Sheet

RBRC10969

Information1

Image
BRC No.RBRC10969
TypeCRISPR/Cas9 (Transgene)Cartagena
SpeciesMus musculus
Strain nameB6(Cg)-T<em1(dre)Okn>
Former Common nameTriple lineagelabelling mice (brachyury2A-Dre Delta Puro)
H-2 Haplotype
ES Cell lineB6NJ-22<UTR> [C57BL/6NCrlCrlj]
Background strain
Appearance
Strain developmentDeposited by Hideyuki Okano, Keio University in 2020. C57BL/6 genetic background.
Strain descriptionKnock-in mice of Dre at the C-terminus of protein coding sequence of the T gene.
Colony maintenance
ReferencesDevelopment, 146(21). pii: dev174938 (2019). 31597657

Health Report

Examination Date / Room / Rack2023/02/27Room:4-BRack:H
2023/02/20Room:4-BRack:H
2022/11/29Room:4-BRack:H
2022/08/29Room:4-BRack:H
2022/05/31Room:4-BRack:H
2022/03/07Room:4-BRack:H
2021/12/06Room:4-BRack:H
2021/09/08Room:4-BRack:H
2021/06/07Room:4-BRack:H

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Tbrachyury17T<em1(dre)Okn>endonuclease-induced mutation 1, Hideyuki Okano

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
DreP1-like transducing phage D6 Dre17Dre

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
F3yeast FRT (flippase recombination target) site (synthetic) variant17F3

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
P2Aporcine teschovirus-1 2A peptide17P2A

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
nlsSV40 large T antigen nuclear localization signal (NLS)17nls

Information2

Donor DNAmouse T/brachyury genomic DNA, P2A (porcine teschovirus-1 2A peptide), P1-like transducing phage D6 Dre, SV40 virus NLS, Saccharomyces cerevisia FRT variant(F3)
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Development. 2019 Nov 4;146(21). pii: dev174938
DepositorHideyuki Okano (Keio University)
Strain Statusan icon for Live miceLive mice
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Live mouse (1 to 3 months)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP

BRC mice in Publications

No Data