Strain Data Sheet

RBRC10968

Strain Information

Image
BRC No.RBRC10968
TypeCRISPR/Cas9 (Transgene)Cartagena
SpeciesMus musculus
Strain nameB6(Cg)-Sox1<em1(cre)Okn>
Former Common nameTriple lineagelabelling mice (Sox12A-Cre Delta Puro)
H-2 Haplotype
ES Cell lineB6NJ-22<UTR> [C57BL/6NCrlCrlj]
Background strain
Appearance
Strain developmentDeposited by Hideyuki Okano, Keio University in 2020. C57BL/6 genetic background.
Strain descriptionKnock-in mice of Cre at the C-terminus of protein coding sequence of the Sox1 gene.
Colony maintenance
ReferencesDevelopment, 146(21). pii: dev174938 (2019). 31597657

Health Report

Examination Date / Room / Rack2023/11/27Room:4-BRack:D
2023/08/28Room:4-BRack:D
2023/05/29Room:4-BRack:D
2023/04/10Room:4-BRack:D
2023/02/27Room:4-BRack:D
2022/11/29Room:4-BRack:D
2022/08/29Room:4-BRack:D
2022/05/31Room:4-BRack:D
2022/03/07Room:4-BRack:D

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Sox1SRY-box containing gene 18Sox1<em1(cre)Okn>endonuclease-induced mutation 1, Hideyuki Okano

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site8Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
P2Aporcine teschovirus-1 2A peptide8P2A

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
crePhage P1 Cre recombinase8cre

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
nlsSV40 large T antigen nuclear localization signal (NLS)8nls

Ordering Information

Donor DNAP2A (porcine teschovirus-1 2A peptide), SV40 virus NLS, bacteriophage P1 Cre, Saccharomyces cerevisia FRT site, mouse Sox1 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Development. 2019 Nov 4;146(21). pii: dev174938
DepositorHideyuki Okano (Keio University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

No Data