Strain Data Sheet

RBRC10967

Information1

Image
BRC No.RBRC10967
TypeCRISPR/Cas9 (Transgene)Cartagena
SpeciesMus musculus
Strain nameB6(Cg)-Sox1<em1(cre)Okn> T<em1(dre)Okn> Sox17<em1(phiC31<*>)Okn>/2-3
Former Common nameTriple lineagelabelling mice (Sox12A-Cre Delta Puro, brachyury2A-Dre Delta Puro, Sox172A-phiC31o Delta Puro)
H-2 Haplotype
ES Cell lineB6NJ-22<UTR> [C57BL/6NCrlCrlj]
Background strain
Appearance
Strain development
Strain description
Colony maintenance
ReferencesDevelopment, 146(21). pii: dev174938 (2019). 31597657

Health Report

Examination Date / Room / Rack2022/03/07Room:4-BRack:B
2021/12/06Room:4-BRack:B
2021/09/08Room:4-BRack:B
2021/06/07Room:4-BRack:B
2021/03/22Room:4-BRack:B
2020/12/14Room:4-BRack:B

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Sox17SRY (sex determining region Y)-box 171Sox17<em1(phiC31<*>)Okn>endonuclease-induced mutation 1, Hideyuki Okano

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
F5yeast FRT (flippase recombination target) site (synthetic) variant1F5

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
P2Aporcine teschovirus-1 2A peptide1P2A

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
PhiC31oPhiC31 recombinase1PhiC31orat tyrosine hydroxylase (TH) promoter

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
nlsSV40 large T antigen nuclear localization signal (NLS)1nls

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Tbrachyury17T<em1(dre)Okn>endonuclease-induced mutation 1, Hideyuki Okano

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
DreP1-like transducing phage D6 Dre17Dre

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
F3yeast FRT (flippase recombination target) site (synthetic) variant17F3

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
P2Aporcine teschovirus-1 2A peptide17P2A

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
nlsSV40 large T antigen nuclear localization signal (NLS)17nls

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Sox1SRY-box containing gene 18Sox1<em1(cre)Okn>endonuclease-induced mutation 1, Hideyuki Okano

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site8Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
P2Aporcine teschovirus-1 2A peptide8P2A

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
crePhage P1 Cre recombinase8cre

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
nlsSV40 large T antigen nuclear localization signal (NLS)8nls

Information2

Donor DNAP2A (porcine teschovirus-1 2A peptide), SV40 virus NLS, bacteriophage P1 Cre, Saccharomyces cerevisia FRT site, mouse Sox1 genomic DNA, porcine teschovirus-1 2A (P2A) peptide, SV40 virus NLS, P1-like transducing phage D6 Dre, SV40 virus NLS, Saccharomyces cerevisia FRT site(F3), mouse T/brachyury genomic DNA, porcine teschovirus-1 2A (P2A) peptide, SV40 virus NLS, Streptomyces lividans phiC31o, Saccharomyces cerevisia FRT site(F5), mouse Sox17 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Development. 2019 Nov 4;146(21). pii: dev174938
DepositorHideyuki Okano (Keio University)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Live mouse (1 to 3 months)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

No Data