Strain Data Sheet

RBRC10967

Strain Information

Image
BRC No.RBRC10967
TypeCRISPR/Cas9 (Transgene)Cartagena
SpeciesMus musculus
Strain nameB6(Cg)-Sox1<em1(cre)Okn> T<em1(dre)Okn> Sox17<em1(phiC31<*>)Okn>/2-3
Former Common nameTriple lineagelabelling mice (Sox12A-Cre Delta Puro, brachyury2A-Dre Delta Puro, Sox172A-phiC31o Delta Puro)
H-2 Haplotype
ES Cell lineB6NJ-22<UTR> [C57BL/6NCrlCrlj]
Background strain
Appearance
Strain developmentDeposited by Hideyuki Okano, Keio University in 2020. C57BL/6 genetic background.
Strain descriptionTriple knockin for three different recombinases: Sox1-Cre, T-Dre and Sox17-PhiC31<*>.All recombinases were knockined at the C-terminus of protein coding sequence of the respective gene allele.
Colony maintenance
ReferencesDevelopment, 146(21). pii: dev174938 (2019). 31597657

Health Report

Examination Date / Room / Rack2023/08/28Room:4-BRack:B
2023/05/29Room:4-BRack:B
2023/02/27Room:4-BRack:B
2022/11/29Room:4-BRack:B
2022/08/29Room:4-BRack:B
2022/05/31Room:4-BRack:B
2022/03/07Room:4-BRack:B

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Sox17SRY (sex determining region Y)-box 171Sox17<em1(phiC31<*>)Okn>endonuclease-induced mutation 1, Hideyuki Okano

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
F5yeast FRT (flippase recombination target) site (synthetic) variant1F5

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
P2Aporcine teschovirus-1 2A peptide1P2A

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
PhiC31oPhiC31 recombinase1PhiC31orat tyrosine hydroxylase (TH) promoter

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
nlsSV40 large T antigen nuclear localization signal (NLS)1nls

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Tbrachyury17T<em1(dre)Okn>endonuclease-induced mutation 1, Hideyuki Okano

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
DreBacteriophage D6 recombinase17Dre

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
F3yeast FRT (flippase recombination target) site (synthetic) variant17F3

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
P2Aporcine teschovirus-1 2A peptide17P2A

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
nlsSV40 large T antigen nuclear localization signal (NLS)17nls

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Sox1SRY-box containing gene 18Sox1<em1(cre)Okn>endonuclease-induced mutation 1, Hideyuki Okano

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site8Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
P2Aporcine teschovirus-1 2A peptide8P2A

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
crePhage P1 Cre recombinase8cre

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
nlsSV40 large T antigen nuclear localization signal (NLS)8nls

Ordering Information

Donor DNAP2A (porcine teschovirus-1 2A peptide), SV40 virus NLS, bacteriophage P1 Cre, Saccharomyces cerevisia FRT site, mouse Sox1 genomic DNA, porcine teschovirus-1 2A (P2A) peptide, SV40 virus NLS, P1-like transducing phage D6 Dre, SV40 virus NLS, Saccharomyces cerevisia FRT site(F3), mouse T/brachyury genomic DNA, porcine teschovirus-1 2A (P2A) peptide, SV40 virus NLS, Streptomyces lividans phiC31o, Saccharomyces cerevisia FRT site(F5), mouse Sox17 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Development. 2019 Nov 4;146(21). pii: dev174938
DepositorHideyuki Okano (Keio University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

No Data