Strain Data Sheet

RBRC10966

Information1

Image
BRC No.RBRC10966
TypeCRISPR/Cas9 (Transgene)Cartagena
SpeciesMus musculus
Strain nameB6(Cg)-Gt(ROSA)26Sor<em1(CAG-venus,-mCherry,-mCerulean)Okn>/26
Former Common nameROSA26<MultiFPs Delta Puro>
H-2 Haplotype
ES Cell lineB6NJ-22<UTR> [C57BL/6NCrlCrlj]
Background strain
Appearance
Strain development
Strain description
Colony maintenance
ReferencesDevelopment, 146(21). pii: dev174938 (2019). 31597657

Health Report

Examination Date / Room / Rack2022/03/14Room:3-3Rack:A
2021/12/14Room:3-3Rack:A
2021/09/13Room:3-3Rack:A
2021/06/14Room:3-3Rack:A
2021/03/29Room:3-3Rack:A
2021/01/05Room:3-3Rack:A

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<em1(CAG-venus,-mCherry,-mCerulean)Okn>endonuclease mediated mutation 1, Hideyuki Okano

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site6Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GHGrowth hormone polyA (Bovine)6GH

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
VenusYellow Fluorescent Protein (Aequorea victoria)6Venus

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
attBlambda phage recombination sites6attB

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
attPlambda phage recombination target site6attP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
mCeruleanCyan Fluorescent protein (Aequorea victoria)6mCerulean

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
mCherryRed Fluorescent Protein (Discosoma sp.)6mCherry

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
puropuromycin resistance gene (Streptomyces alboniger)6puroCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
roxD6 Bacteriophage rox site6rox

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
roxD6 Bacteriophage rox site6rox

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA signal6

Information2

Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), bacteriophage P1 loxP sites, Streptomyces lividans attB site, P1-like transducing phage D6 rox sites, Streptomyces alboniger Puromycin resistance gene, jellyfish GFP variant (Venus) cDNA, Entacmaea quadricolor RFP variant (mCherry) cDNA, SV40 polyA, Streptomyces lividans attP site, jellyfish GFP variant (mCerulean)cDNA, bovine growth hormone polyA, Saccharomyces cerevisia FRT site, mouse Gt(ROSA)26Sor genomic DNA
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Development. 2019 Nov 4;146(21). pii: dev174938
DepositorHideyuki Okano (Keio University)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Live mouse (1 to 3 months)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  3. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEVELOPER of the Venus using Approval form (Form V (English)) (Lab Contact: Laboratory for Cell Function Dynamics, RIKEN CBS: mta-cfds@ml.riken.jp).
  4. CAGGS MTA (English)
  5. Acceptance of responsibility for living modified organism (Japanese / English)
  6. GFP Transfer License (Japanese / English)
    Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.
Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

No Data