Strain Data Sheet

RBRC10965

Information1

Image
BRC No.RBRC10965
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameFVB-Tns2<em2Nsas>
Former Common nameFVB-Tns2<C231S>
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Nobuya Sasaki, Kitasato University in 2016. This strain was generated by the CRISPR/Cas9 using eggs derived from FVB/NJ(FVB). FVB/NJ(FVB) genetic background.
Strain descriptionTns2 mutants harboring amino acid substitution of C231S.
Colony maintenance
ReferencesExp. Anim., (2019). 31723089

Health Report

Examination Date / Room / Rack2022/03/14Room:3-5Rack:D
2021/12/14Room:3-5Rack:D
2021/09/13Room:3-5Rack:D
2021/06/14Room:3-5Rack:D
2021/03/29Room:3-5Rack:D
2021/01/05Room:3-5Rack:D

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Tns2tensin 215Tns2<em2Nsas>endonuclease-mediated mutation 2, Nobuya Sasaki

Information2

Donor DNAS. pyogenes Single guide RNA, S. pyogenes Humanized Cas9* gene mRNA, Mouse Tns2 gene RNA flagment (Single quide RNA), Mouse Mutant Tns2 gene single-strand ODN* Not detected by PCR using Marker Gene Detection kit (TOYOBO, Osaka, Japan). Other introduced genes were not tested.
Research application
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. Depending on the contact of the research, the DEPOSITOR may request collaboration.
DepositorNobuya Sasaki (Kitasato University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data