RBRC10964

Information1

Image
BRC No.RBRC10964
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameB6-Kcnh7<em1Nsas>
Former Common nameB6-Kcnh7 N64
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain development
Strain description
Colony maintenance
References

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Kcnh7potassium voltage-gated channel, subfamily H (eag-related), member 72Kcnh7<em1Nsas>endonuclease-mediated mutation 1, Nobuya Sasaki

Information2

Donor DNAS. pyogenes Single guide RNA, S. pyogenes Humanized Cas9* gene, Mouse Kcnh7 gene RNA flagment (Single guide RNA), CMV CMV enhancer DNA, Chicken Actb gene promoter DNA, MMV MMV intron DNA, SV40 Nuclear localization signal DNA, Bovine Growth hormone polyA signal DNA, AAV2 Inverted terminal repeat DNA, f1 phage Origin of replication DNA, E. coli AmpR* gene promoter and cDNA, E. coli Origin of replication DNA, Human RNA polymerase III promoter DNA
Research application
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. Depending on the contact of the research, the DEPOSITOR may request collaboration.
DepositorNobuya Sasaki (Kitasato University)
Strain Statusan icon for Live miceLive mice
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-
Category I MTA: CRISPR/Cas9 genome edited bioresources

BRC mice in Publications

No Data