Strain Data Sheet

RBRC10964

Information1

Image
BRC No.RBRC10964
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameB6-Kcnh7<em1Nsas>
Former Common nameB6-Kcnh7 N64
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Nobuya Sasaki, Kitasato University in 2016. This strain was generated by the CRISPR/Cas9 using eggs derived from C57BL/6J. C57BL/6J genetic background.
Strain descriptionKcnh7 mutants lacking 64 amino acid at N-terminal cyotoplasmic tail.
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Kcnh7potassium voltage-gated channel, subfamily H (eag-related), member 72Kcnh7<em1Nsas>endonuclease-mediated mutation 1, Nobuya Sasaki

Information2

Donor DNAS. pyogenes Single guide RNA, S. pyogenes Humanized Cas9* gene, Mouse Kcnh7 gene RNA flagment (Single guide RNA), CMV enhancer DNA, Chicken Actb gene promoter DNA, MMV MMV intron DNA, SV40 Nuclear localization signal DNA, Bovine Growth hormone polyA signal DNA, AAV2 Inverted terminal repeat DNA, f1 phage Origin of replication DNA, E. coli AmpR** gene promoter and cDNA, E. coli Origin of replication DNA, Human RNA polymerase III promoter DNA* Not detected by PCR using Marker Gene Detection kit (TOYOBO, Osaka, Japan). ** Not detected by PCR. Other introduced genes were not tested.
Research application
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. Depending on the contact of the research, the DEPOSITOR may request collaboration.
DepositorNobuya Sasaki (Kitasato University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data