Strain Data Sheet

RBRC10958

Strain Information

Image
BRC No.RBRC10958
TypeCRISPR/Cas9 (Transgene)Cartagena
SpeciesMus musculus
Strain nameC57BL/6-Gcg<em1(cre)Utr>
Former Common nameGcg-Cre
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Satoru Takahashi, University of Tsukuba in 2020. C57BL/6J genetic background.
Strain descriptionCre recombinase was placed at 3’ termini of protein coding of the Gcg gene via 2A peptide.
Colony maintenance
References
Uncovering the role of MAFB in glucagon production and secretion in pancreatic α-cells using a new α-cell-specific Mafb conditional knockout mouse model.
Yu-Hsin Chang, Megumi C Katoh, Ahmed M Abdellatif, Guli Xiafukaiti, Abdelaziz Elzeftawy, Masami Ojima, Seiya Mizuno, Akihiro Kuno, Satoru Takahashi
Exp. Anim., 69(2):178-188 (2020). 31787710

Health Report

Examination Date / Room / Rack2025-08-18Room:3-3Rack:ASentinel mouse program
2025-06-09Room:3-3Rack:ASentinel mouse program
2025-03-10Room:3-3Rack:ASentinel mouse program
2024-12-09Room:3-3Rack:ASentinel mouse program
2024-09-09Room:3-3Rack:ASentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Gcg
MGI:95674		glucagon2Gcg<em1(cre)Utr> endonuclease-induced mutation 1, University of Tsukuba Laboratory Animal Resource Center
HBB2 rabbit bata-globin polyA2HBB2
P2A porcine teschovirus-1 2A peptide2P2A
cre Phage P1 Cre recombinase2cre
nls SV40 large T antigen nuclear localization signal (NLS)2nls

Phenotype

Phenotype annotation from literatures by Mammalian phenotype ontology
  • normal phenotype (MP:0002873)
    • Detailed phenotype data

    Ordering Information

    Donor DNAP2A (porcine teschovirus-1 2A peptide), SV40 nls, P1 phage Cre recombinase, rabbit beta-globin polyA
    Research applicationCre/loxP system
    Cre driver
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Exp. Anim., 69(2):178-188 (2020).
    DepositorSatoru Takahashi (University of Tsukuba)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    Tsuno T, Li J, Nishiyama K, Kawasawa YI, Inoue R, Ong Yajima E, Nishiyama A, Yabe SG, Kin T, Okochi H, Tamura T, Shapiro AMJ, Oyadomari S, Kitamura T, Terauchi Y, Shirakawa J.
    Imeglimin suppresses glucagon secretion and induces a loss of α cell identity.
    Cell Rep Med 102254(2025) 40713970