Strain Data Sheet

RBRC10958

Information1

Image
BRC No.RBRC10958
TypeCRISPR/Cas9 (Transgene)Cartagena
SpeciesMus musculus
Strain nameC57BL/6-Gcg<em1(cre)Utr>
Former Common nameGcg-Cre
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Satoru Takahashi, University of Tsukuba in 2020. C57BL/6J genetic background.
Strain descriptionCre recombinase was placed at 3’ termini of protein coding of the Gcg gene via 2A peptide.
Colony maintenance
ReferencesExp. Anim., (2019). doi: 10.1538/expanim.19-0105. 31787710

Health Report

Examination Date / Room / Rack2023/09/11Room:3-3Rack:A
2023/06/13Room:3-3Rack:A
2023/03/13Room:3-3Rack:A
2022/12/12Room:3-3Rack:A
2022/09/12Room:3-3Rack:A
2022/06/13Room:3-3Rack:A
2022/03/14Room:3-3Rack:A
2021/12/14Room:3-3Rack:A

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Gcgglucagon2Gcg<em1(cre)Utr>endonuclease-induced mutation 1, University of Tsukuba Laboratory Animal Resource Center

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
HBB2rabbit bata-globin polyA2HBB2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
P2Aporcine teschovirus-1 2A peptide2P2A

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
crePhage P1 Cre recombinase2cre

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
nlsSV40 large T antigen nuclear localization signal (NLS)2nls

Information2

Donor DNAP2A (porcine teschovirus-1 2A peptide), SV40 nls, P1 phage Cre recombinase, rabbit beta-globin polyA
Research applicationCre/loxP system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Exp Anim. 2019 Dec 2. doi: 10.1538/expanim.19-0105.
DepositorSatoru Takahashi (University of Tsukuba)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Live mouse (1 to 3 months)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data