Strain Data Sheet
RBRC10958
Strain Information | |
|---|---|
| Image | |
| BRC No. | RBRC10958 |
| Type | CRISPR/Cas9 (Transgene) |
| Species | Mus musculus |
| Strain name | C57BL/6-Gcg<em1(cre)Utr> |
| Former Common name | Gcg-Cre |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | |
| Appearance | |
| Strain development | Deposited by Satoru Takahashi, University of Tsukuba in 2020. C57BL/6J genetic background. |
| Strain description | Cre recombinase was placed at 3’ termini of protein coding of the Gcg gene via 2A peptide. |
| Colony maintenance | |
| References | Uncovering the role of MAFB in glucagon production and secretion in pancreatic alpha-cells using a new alpha-cell-specific Mafb conditional knockout mouse model. Chang Y H, Katoh M C, Abdellatif A M, Xiafukaiti G, Elzeftawy A, Ojima M, Mizuno S, Kuno A, Takahashi S Exp. Anim., 69(2):178-188 (2020). 31787710 |
Health Report | |
|---|---|
| Examination Date / Room / Rack | 2025/08/18Room:3-3Rack:ASentinel mouse program 2025/06/09Room:3-3Rack:ASentinel mouse program 2025/03/10Room:3-3Rack:ASentinel mouse program 2024/12/09Room:3-3Rack:ASentinel mouse program 2024/09/09Room:3-3Rack:ASentinel mouse program 2024/06/10Room:3-3Rack:ASentinel mouse program 2024/03/11Room:3-3Rack:ASentinel mouse program |
Gene | |||||||
|---|---|---|---|---|---|---|---|
| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| Gcg MGI:95674 | glucagon | 2 | Gcg<em1(cre)Utr> | endonuclease-induced mutation 1, University of Tsukuba Laboratory Animal Resource Center | |||
| HBB2 | rabbit bata-globin polyA | 2 | HBB2 | ||||
| P2A | porcine teschovirus-1 2A peptide | 2 | P2A | ||||
| cre | Phage P1 Cre recombinase | 2 | cre | ||||
| nls | SV40 large T antigen nuclear localization signal (NLS) | 2 | nls | ||||
Phenotype | |
|---|---|
| Phenotype annotation from literatures by Mammalian phenotype ontology | |
| Detailed phenotype data | |
Ordering Information | |
|---|---|
| Donor DNA | P2A (porcine teschovirus-1 2A peptide), SV40 nls, P1 phage Cre recombinase, rabbit beta-globin polyA |
| Research application | Cre/loxP system Cre driver |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Exp. Anim., 69(2):178-188 (2020). |
| Depositor | Satoru Takahashi (University of Tsukuba) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
|---|
Tsuno T, Li J, Nishiyama K, Kawasawa YI, Inoue R, Ong Yajima E, Nishiyama A, Yabe SG, Kin T, Okochi H, Tamura T, Shapiro AMJ, Oyadomari S, Kitamura T, Terauchi Y, Shirakawa J. Imeglimin suppresses glucagon secretion and induces a loss of α cell identity. Cell Rep Med 102254(2025) 40713970 |