Strain Data Sheet

RBRC10946

Strain Information

Image
BRC No.RBRC10946
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Alpl<tm1(cre)Nagy>/Evo
Former Common nameTNAP-Cre
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearance
Strain developmentDeposited by Kazyo Kinoshita, Shiga Medical Center Research Institute in 2019. This strain was backcrossed to C57BL/6J for 20 generations.
Strain descriptionCre knockin mice at the TNAP (Alpl) locus.
Colony maintenance
ReferencesInt. Immunol., 33(7):387-398 (2021). 33903914
Genesis, 26(2):116-7 (2000). 10686602

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Alplalkaline phosphatase, liver/bone/kidney4Alpl<tm1(cre)Nagy>

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
IRESinternal ribosomal entry site (EMCV)4

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
crePhage P1 Cre recombinase4cre

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)4mouse phosphoglycerate kinase promoter (PGK promoter)

Ordering Information

Donor DNAencephalomyocarditis virus IRES, Bacteriophage P1 Cre gene, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistant gene, unknown Pgk1 PolyA signal
Research applicationCre/loxP system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genesis, 26(2):116-7 (2000). Int Immunol., 33(7):387-398 (2021).For for-profit institutions - please contact Sinai Health System for a license : Michael Hanna, Sr. Busmers Officer hanna@lunenfeld.ca
DepositorKazuo Kinoshita (Shiga Medical Center Research Institute)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data