Strain Data Sheet

RBRC10943

Information1

Image
BRC No.RBRC10943
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Aicda<tm1.1Evo>
Former Common nameAicda-RFP
H-2 Haplotype
ES Cell lineBruce 4 [B6.Cg-Thy1<a>]
Background strain
Appearance
Strain developmentDeveloped by Kazuo Kinoshita, Shiga Medical Center Research Institute in 2019. Bruce 4 ES cells were used. C57BL/6 congenic line.
Strain descriptionRFP reporter mice for the Aicda gene. loxP-RFP-frt-Neo-frt-loxP DNA fragment was placed upstream of Exon 2 and another loxP was placed downstream of Exon 3.
Colony maintenance
ReferencesInt. Immunol., 33(7):387-398 (2021). 33903914

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Aicdaactivation-induced cytidine deaminase6Aicda<tm1.1Evo>targeted mutation 1.1, Kazuo Kinoshita

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site6Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
tdRFPtandem-dimer Red Fluorescent Protein (Discosoma sp.)6tdRFP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA signal6

Information2

Donor DNABacteriophage P1 loxP sites, Coral of the Discosoma genus tdRFP gene, SV40 PolyA signal, S. cerevisiae frt site, mouse Aicda genomic DNA
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Int Immunol. 2021 Jun 18;33(7):387-398. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
DepositorKazuo Kinoshita (Shiga Medical Center Research Institute)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. UCSD MTA
  4. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data