Strain Data Sheet

RBRC10942

Information1

Image
BRC No.RBRC10942
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameC57BL/6J-Trps1<em1Osud>
Former Common nameTrps1 Enhancer1 knockout mouse
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Makoto Abe, Osaka University in 2019. This strain was generated by the Advanced Animal model Support. Mutant shows mild scoliosis.
Strain descriptionMutant mice lacking about 3 kb genomic fragment upstream the Trps1 gene.
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Trps1transcriptional repressor GATA binding 115Trps1<em1Osud>endonuclease-mediated mutation 1, Makoto Abe

Information2

Donor DNA[Streptococcus Pyogenes Cas9*, Simian Virus SV40 NLS, Porcine teschovirus 2A peptide sequence, Streptomyces alboniger puromycin resistance gene*, Bos taurus bGH polyA, E. coli ampicilin resistance gene, human U6 promoter, CMV enhancer, Streptococcus Pyogenes trscrRNA, mouse gRNA sequence]* Not detected by PCR using Marker Gene Detection kit (TOYOBO, Osaka, Japan). Other introduced genes were not tested.
Research application
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. 後日指定RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
DepositorMakoto Abe (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data