Strain Data Sheet

RBRC10939

Information1

Image
BRC No.RBRC10939
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameC57BL/6N-Slc38a4<em2Smtb>
Former Common nameSlc38a4 KO
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped and deposited by Shogo Matoba and Atsuo Ogura at RIKEN BRC in 2019. This strain was generated by the CRISPR/Cas9 using fertilized eggs derived from C57BL/6N.
Strain descriptionSlc38a4 mutant strain (line B).
Colony maintenance
ReferencesProc. Natl. Acad. Sci. USA, 116(42):21047-21053 (2019). 31570606

Health Report

Examination Date / Room / Rack2022/04/25Room:3-CRack:J
2022/02/14Room:3-CRack:J
2021/11/15Room:3-CRack:J
2021/08/16Room:3-CRack:J
2021/05/17Room:3-CRack:J
2021/02/15Room:3-CRack:J
2020/11/16Room:3-CRack:J

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Slc38a4solute carrier family 38, member 415Slc38a4<em2Smtb>endonuclease-mediated mutation 2, Shogo Matoba

Information2

Donor DNAStreptococcus pyogenes Cas9* nuclease (human codon optimized), Streptococcus pyogenes tracrRNA(in the form of sgRNA) * Not detected by PCR using Marker Gene Detection kit (TOYOBO, Osaka, Japan). Other introduced genes were not tested.
Research application
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. USA, doi: 10.1073/pnas.1907884116.In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
DepositorAtsuo Ogura (RIKEN)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved embryos (within 1 month)
Additional Info.Genotyping protocol -PCR-
Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP

BRC mice in Publications

No Data