Strain Data Sheet

RBRC10928

Information1

Image
BRC No.RBRC10928
TypeCRISPR/Cas9 (Transgene)Cartagena
SpeciesMus musculus
Strain nameC57BL/6J-Ucp1<em1(iRFP720)Utgr>
Former Common nameUcp1-iRFP720 KI mice
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Aya Fukuda, University of Tsukuba in 2017. This strain was generated with the CRISPR/Cas9 using fertilized eggs derived from C57BL/6J. Homozygous mice are fertile.
Strain descriptionA reporter strain in which iRFP720 is placed around the translation initiation codon of the Ucp1 gene.
Colony maintenance
References

Health Report

Examination Date / Room / Racknull

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Ucp1uncoupling protein 1 (mitochondrial, proton carrier)8Ucp1<em1(iRFP720)Utgr>endonuclease-mediated mutation 1, Koji Hisataka

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GHGrowth hormone polyA (Bovine)8GH

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
HBBhuman beta-globin intron8HBB

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
iRFP720Deinococcus radiodurans8iRFP720

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
human IgH intron8

Information2

Donor DNApX330-U6-Chimeric_BB-CBh-hSpCas9[human U6 promoter, S. pyogenes gRNA scaffold, human U6 terminator, CMV,chicken hybrid CMV enhancer/chicken beta-actin promotor (CBh), Synthetic DNA 3xFLAG, SV40 nuclear localization signal(NLS), Streptococcus pyogenes SpCas9 (human codon-optimized), bovine GH polyA signal, AAV2 inverted terminal repeat (ITR), f1 phage f1 origin, E. coli Ampicillin resistance gene (AmpR), E. coli pUC origin], Mus musculus Ucp1 genomic DNA, Homo sapiens IgH intron, Homo sapiens beta-globin intron, Mus musculus Kozak sequence, Deinococcus radiodurans iRFP720, Bos taurus bGH polyA
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. 後日指定
DepositorAya Fukuda (University of Tsukuba)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Mouse of the Month Feb 2021
Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data