RBRC10928
Information1 | |
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| Image | |
| BRC No. | RBRC10928 |
| Type | CRISPR/Cas9 (Transgene) |
| Species | Mus musculus |
| Strain name | C57BL/6J-Ucp1<em1(iRFP720)Utgr> |
| Former Common name | Ucp1-iRFP720 KI mice |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | |
| Appearance | |
| Strain development | |
| Strain description | |
| Colony maintenance | |
| References | |
Health Report | |
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| Examination Date / Room / Rack | No Data |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter GHGrowth hormone polyA (Bovine)8GH Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter HBB2human beta-globin intron8HBB2 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter iRFP720Deinococcus radiodurans8iRFP720 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter human IgH intron8 |
Information2 | |
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| Donor DNA | pX330-U6-Chimeric_BB-CBh-hSpCas9[human U6 promoter, S. pyogenes gRNA scaffold, human U6 terminator, CMV,chicken hybrid CMV enhancer/chicken beta-actin promotor (CBh), Synthetic DNA 3xFLAG, SV40 nuclear localization signal(NLS), Streptococcus pyogenes SpCas9 (human codon-optimized), bovine GH polyA signal, AAV2 inverted terminal repeat (ITR), f1 phage f1 origin, E. coli Ampicillin resistance gene (AmpR), E. coli pUC origin], Mus musculus Ucp1 genomic DNA, Homo sapiens IgH intron, Homo sapiens beta-globin intron, Mus musculus Kozak sequence, Deinococcus radiodurans iRFP720, Bos taurus bGH polyA |
| Research application | Fluorescent Proteins/lacZ System |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. 後日指定 |
| Depositor | Aya Fukuda (University of Tsukuba) |
| Strain Status |  Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
| Additional Info. | Category I MTA: CRISPR/Cas9 genome edited bioresources ( Japanese / English ) |
BRC mice in Publications |
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