Strain Data Sheet

RBRC10926

Information1

Image
BRC No.RBRC10926
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Hcn4<tm1.1(Luc/EGFP)Nna>
Former Common nameHCN4 +/luc mice
H-2 Haplotype
ES Cell lineBruce 4 [B6.Cg-Thy1<a>]
Background strain
Appearance
Strain developmentDeveloped by Makoto Takano, Kurume University in 2015. Bruce 4 ES cells were used. C57BL/6 congenic strain.
Strain descriptionReporter strain in which luciferase/EGFP fusion gene was inserted in frame of translation initiation codon of the Hcn4 gene. Hcn4 gene is disrupted.
Colony maintenance
ReferencesJ. Physiol., 596(5):809-825 (2018). 29315578

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Hcn4hyperpolarization-activated, cyclic nucleotide-gated K+ 49Hcn4<tm1.1(Luc/EGFP)Nna>targeted mutation 1, Noriyuki Nakashima

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)9EGFP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP9loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lucluciferase9luc

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA signal9

Information2

Donor DNAfirefly Luciferase cDNA(U47298), jellyfish EGFP cDNA(U03453.1), Simian virus SV40 pA, P1 phage loxP site, mouse Hcn4 genomic DNA
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Physiol., 596(5):809-825 (2018).For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
DepositorMakoto Takano (Kurume University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)
  4. GFP Transfer License (Japanese / English)
    Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

BRC mice in Publications

No Data