Strain Data Sheet

RBRC10923

Strain Information

Image
BRC No.RBRC10923
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.B6D2-Tg(Krt19-icre)2Hirt
Former Common nameKrt19 iCre
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Hiroyuki Tomita, Gifu University in 2019. A BAC transgenic DNA was injected to fertilized eggs derived from BDF mice. Mice were further backcrossed to C57B6/J for 10 generations. C57B6/J congenic strain.
Strain descriptionBAC transgenic mice expressing iCre under the control of Krt19 gene promoter.
Colony maintenance
References
Characterization of a BAC transgenic mouse expressing Krt19-driven iCre recombinase in its digestive organs.
Tomohiro Kanayama, Hiroyuki Tomita, Nguyen Huy Binh, Yuichiro Hatano, Hitomi Aoki, Hideshi Okada, Akihiro Hirata, Yoshitaka Fujihara, Takahiro Kunisada, Akira Hara
PLoS One, 14(8):e0220818 (2019). 31393940

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) siteUNFrt
icre phage P1 codon improved Cre (iCre)UNicre mouse Krt19 promoter
Simian virus SV40 polyAUN

Phenotype

Phenotype annotation from literatures by Mammalian phenotype ontology
  • no abnormal phenotype detected (MP:0002169)
    • Detailed phenotype data

    Ordering Information

    Donor DNAmouse Krt19 genomic DNA, phage P1 iCre (improved Cre), yeast FRT (flippase recombination target) site, Simian virus SV40 polyA
    Research applicationCre/loxP system
    FLP/frt system
    Cre driver
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. PLoS One, 14(8):e0220818 (2019).
    DepositorHiroyuki Tomita (Gifu University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data