Strain Data Sheet

RBRC10920

Information1

Image
BRC No.RBRC10920
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Gt(ROSA)26Sor<tm1(mVenus/Cdkn1b<*>)Toki>
Former Common nameG0 marker mice
H-2 Haplotype
ES Cell lineKY1.1[(C57BL/6J x 129S6/SvEvTac)F1]
Background strain
Appearance
Strain developmentDeveloped and deposited by Toshio Kitamura in 2019. ES cells derived from (C57BL/6J x 129S6/SvEvTac)F1 were used. Mice were further backcrossed to C57BL/6J for 10 generations. C57BL/6J congenic line.
Strain descriptionKnockin mouse strain at the ROSA26 locus in which mVenus-fused p27K can be overexpressed in a Cre-dependent manner.
Colony maintenance
ReferencesCell Rep., 29(12):4144-4158 (2019). 31851939

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Cdkn1bcyclin-dependent kinase inhibitor 1B6Cdkn1b

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm1(mVenus/Cdkn1b<*>)Toki>targeted mutation 1, Toshio Kitamura

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SAadenovirus Splicing acceptor6SA

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
mVenusYellow Fluorescent Protein (Aequorea victoria)6mVenus

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)6herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA signal6

Information2

Donor DNAadenovirus Splicing acceptor, P1 phage LoxP sites, herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli Neomycin resistant gene, HSV tk polyA, Aequorea victoria mVenus cDNA, mouse p27K-(mutant p27) gene cDNA, SV40 virus PolyA signal, mouse Gt(ROSA)26Sor genomic DNA
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. 後日指定
DepositorToshio Kitamura (The University of Tokyo)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEVELOPER of the Venus using Approval form (Form V (English)) (Lab Contact: Laboratory for Cell Function Dynamics, RIKEN CBS: mta-cfds@ml.riken.jp).
  5. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data