Strain Data Sheet

RBRC10908

Information1

Image
BRC No.RBRC10908
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.Cg-c/c Tg(Hdc-GFP)1Mpu
Former Common nameTg Hdc-GFP line1
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Takashi Moriguchi, Tohoku Medical and Pharmaceutical University in 2019. A BAC TG construct was injected to fertilized eggs derived from B6D2F1. Mice were backcrossed to a B6 albino strain for 4 generations.
Strain descriptionBAC transgenic mice harboring GFP reporter under the control of Hdc(histidine decarboxylase) gene. Albino strain.
Colony maintenance
ReferencesSci. Rep., 9(1):15603 (2019). 31666556

Health Report

Examination Date / Room / Rack2021/03/29Room:4-CRack:I

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Tyrtyrosinase7Tyr<c-2J>albino 2 Jackson

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) siteUNFrt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GFPGreen Fluorescent Protein (Aequorea victoria)UN

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxPUNloxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxPUNloxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyAUN

Information2

Donor DNAmouse histidine decarboxylase genomic DNA (BAC clone RP23-40N15), jellyfish GFP cDNA, SV40 polyA, Saccharomyces cerevisiae FRT site, E. coli chloramphenicol resistance gene, Phage P1 loxP sites derived from pBACe3.6 vector
Research applicationFLP/frt system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Sci Rep. 2019 Oct 30;9(1):15603.Depending on the content of the research, the DEPOSITOR may request collaboration. The RECIPIENT must contact the DEPOSITOR in the case of a application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorTakashi Moriguchi (Tohoku Pharmaceutical University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
  5. GFP Transfer License (Japanese / English)
    Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

Genotyping protocol -PCR-

BRC mice in Publications

No Data