Strain Data Sheet

RBRC10906

Information1

Image
BRC No.RBRC10906
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-AI847159<tm1Tfur>
Former Common nameB6-D16-Het
H-2 Haplotype
ES Cell lineJM8A3 [C57BL/6N-A<tm1Brd>]
Background strain
Appearance
Strain developmentDeveloped by Takahisa Furukawa, Institute for Protein Research, Osaka University in 2015. JM8A3 ES cells derived from C57BL/6N were used and mice were crossed with C57BL/6J. C57BL/6 genetic background.
Strain descriptionKO mouse strain for the AI847159 gene. A DNA fragment of FRT-pgk-neo-FRT-loxP replaced exon 1, 2 and 3 of the AI847159 gene.
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
AI847159expressed sequence AI8471592AI847159<tm1Tfur>targeted mutation 1, Takahisa Furukawa

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site2Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site2Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP2loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)2mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 PolyA signal2

Information2

Donor DNAYeast FRT sites, Mouse phosphoglycerate kinase promoter, E.coli Neomycin resistance gene, SV40 PolyA signal, Bacteriophage P1 loxP site, Mouse AI847159 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsIn publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT which wants to use the BIOLOGICAL RESOURCE is requested to contact the depositor (Dr. Takahisa Furukawa) and enter into a Material Transfer Agreement with Osaka University.
DepositorTakahisa Furukawa (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data