Strain Data Sheet

RBRC10898

Strain Information

Image
BRC No.RBRC10898
TypeCRISPR/Cas9 (Transgene)Cartagena
SpeciesMus musculus
Strain nameC57BL/6J-Sox17<em1(EGFP)Utr>
Former Common nameSox17-2A-EGFP
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Fumihiro Sugiyama, University of Tsukuba in 2017. This strain was generated with the CRISPR/Cas9 using fertilized eggs derived from C57BL/6J. Homozygous mice are grossly normal and fertile.
Strain descriptionKnockin mice harboring EGFP at the end of translation initiation codon of the Sox17 gene with the P2A peptide.
Colony maintenance
References
Generation of bicistronic reporter knockin mice for visualizing germ layers.
Suzuki H, Dinh T T H, Daitoku Y, Tanimoto Y, Kato K, Azami T, Ema M, Murata K, Mizuno S, Sugiyama F
Exp. Anim., 68(4):499-509 (2019). 31189761

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
EGFP Enhanced Green Fluorescent Protein (Aequorea victoria)1EGFP
HBB2 rabbit beta-globin polyA1HBB2
P2A porcine teschovirus-1 2A peptide1P2A
Sox17
MGI:107543		SRY (sex determining region Y)-box 171Sox17<em1(EGFP)Utr> endonuclease-induced mutation 1, University of Tsukuba Laboratory Animal Resource Center
  • vesicoureteral reflux 3(MedGEN)

    • Phenotype

    Phenotype annotation from literatures by Mammalian phenotype ontology
    Detailed phenotype data

    Ordering Information

    Donor DNAP2A (porcine teschovirus-1 2A peptide), Aequorea victoria Enhanced Green Fluorescent Protein cDNA, Rabbit Beta globin polyA
    Research applicationFluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Exp. Anim., 68(4):499-509 (2019).
    DepositorFumihiro Sugiyama (University of Tsukuba)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data