Strain Data Sheet

RBRC10898

Information1

Image
BRC No.RBRC10898
TypeCRISPR/Cas9 (Transgene)Cartagena
SpeciesMus musculus
Strain nameC57BL/6J-Sox17<em1(EGFP)Utr>
Former Common nameSox17-2A-EGFP
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Fumihiro Sugiyama, University of Tsukuba in 2017. This strain was generated with the CRISPR/Cas9 using fertilized eggs derived from C57BL/6J. Homozygous mice are grossly normal and fertile.
Strain descriptionKnockin mice harboring EGFP at the end of translation initiation codon of the Sox17 gene with the P2A peptide.
Colony maintenance
ReferencesExp. Anim., 2019 Jun 13. doi: 10.1538/expanim.19-0031. 31189761

Health Report

Examination Date / Room / Rack2022/01/24Room:3-BRack:D
2021/10/26Room:3-BRack:D
2021/07/26Room:3-BRack:D
2021/04/26Room:3-BRack:D
2021/02/01Room:3-BRack:D
2020/11/09Room:3-BRack:D

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Sox17SRY (sex determining region Y)-box 171Sox17<em1(EGFP)Utr>endonuclease-induced mutation 1, University of Tsukuba Laboratory Animal Resource Center

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)1EGFP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
HBB2rabbit beta-globin polyA1HBB2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
P2Aporcine teschovirus-1 2A peptide1P2A

Information2

Donor DNAP2A (porcine teschovirus-1 2A peptide), Aequorea victoria Enhanced Green Fluorescent Protein cDNA, Rabbit Beta globin polyA
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Exp. Anim., 2019 Jun 13. doi: 10.1538/expanim.19-0031.
DepositorFumihiro Sugiyama (University of Tsukuba)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-
Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)
  4. GFP Transfer License (Japanese / English)
    Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

BRC mice in Publications

No Data