Strain Data Sheet

RBRC10897

Information1

Image
BRC No.RBRC10897
TypeCRISPR/Cas9 (Transgene)Cartagena
SpeciesMus musculus
Strain nameC57BL/6J-Otx2<em1(tdTomato)Utr>
Former Common nameOtx2-2A-tdTomato
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Fumihiro Sugiyama, University of Tsukuba in 2017. This strain was generated with the CRISPR/Cas9 using fertilized eggs derived from C57BL/6J. Homozygous mice are grossly normal and fertile.
Strain descriptionKnockin mice harboring tdTomato at the end of translation initiation codon of the Otx2 gene with the P2A peptide.
Colony maintenance
ReferencesExp. Anim., 2019 Jun 13. doi: 10.1538/expanim.19-0031. 31189761

Health Report

Examination Date / Room / Rack2022/06/13Room:3-6Rack:B
2022/03/14Room:3-6Rack:B
2021/12/14Room:3-6Rack:B
2021/09/13Room:3-6Rack:B

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Otx2orthodenticle homolog 2 (Drosophila)14Otx2<em1(tdTomato)Utr>endonuclease-induced mutation 1, University of Tsukuba Laboratory Animal Resource Center

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
HBB2rabbit beta-globin polyA14HBB2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
P2Aporcine teschovirus-1 2A peptide14P2A

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
tdTomatotandem dimeric Red Fluorescent Protein (Discosoma sp.)14tdTomato

Information2

Donor DNAP2A (porcine teschovirus-1 2A peptide), Discosoma sp. tdTomato cDNA, Rabbit Beta globin polyA
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Exp. Anim., 2019 Jun 13. doi: 10.1538/expanim.19-0031.
DepositorFumihiro Sugiyama (University of Tsukuba)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Live mouse (1 to 3 months)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data