Strain Data Sheet

RBRC10897

Strain Information

Image
BRC No.RBRC10897
TypeCRISPR/Cas9 (Transgene)Cartagena
SpeciesMus musculus
Strain nameC57BL/6J-Otx2<em1(tdTomato)Utr>
Former Common nameOtx2-2A-tdTomato
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Fumihiro Sugiyama, University of Tsukuba in 2017. This strain was generated with the CRISPR/Cas9 using fertilized eggs derived from C57BL/6J. Homozygous mice are grossly normal and fertile.
Strain descriptionKnockin mice harboring tdTomato at the end of translation initiation codon of the Otx2 gene with the P2A peptide.
Colony maintenance
References
Generation of bicistronic reporter knockin mice for visualizing germ layers.
Suzuki H, Dinh T T H, Daitoku Y, Tanimoto Y, Kato K, Azami T, Ema M, Murata K, Mizuno S, Sugiyama F
Exp. Anim., 68(4):499-509 (2019). 31189761

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
HBB2 rabbit beta-globin polyA14HBB2
Otx2
MGI:97451		orthodenticle homeobox 214Otx2<em1(tdTomato)Utr> endonuclease-induced mutation 1, University of Tsukuba Laboratory Animal Resource Center
  • pituitary hormone deficiency, combined, 6(MedGEN)

  • syndromic microphthalmia type 5(MedGEN)
    • P2A porcine teschovirus-1 2A peptide14P2A
    tdTomato tandem dimeric Red Fluorescent Protein (Discosoma sp.)14tdTomato

    Phenotype

    Phenotype annotation from literatures by Mammalian phenotype ontology
    Detailed phenotype data

    Ordering Information

    Donor DNAP2A (porcine teschovirus-1 2A peptide), Discosoma sp. tdTomato cDNA, Rabbit Beta globin polyA
    Research applicationFluorescent Proteins/lacZ System
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Exp. Anim., 68(4):499-509 (2019).
    DepositorFumihiro Sugiyama (University of Tsukuba)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: CRISPR/Cas9 genome edited bioresources (TBUSAMTA_Nonprofit_BROAD)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data