Strain Data Sheet

RBRC10895

Information1

Image
BRC No.RBRC10895
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129S-Gjc1<tm2.3Kni>
Former Common nameCx45 floxed; Cx45 flox; Connexin45 floxed; Connexin45 flox
H-2 Haplotype
ES Cell lineCCE/EK.CCE [129S/SvEv-Gpi1<c>]
Background strain
Appearance
Strain developmentDeposited by Kiyomasa Nishii, National Defense Medical College in 2019. CCE ES cell line derived from 129/SvEv was used. Crossing with a Cre strain removed a floxed neo selection cassette, but left the floxed entire coding exon of the Cx45 gene.
Strain descriptionFloxed Cx45 (Gjc1; gap junction protein, gamma 1). The entire coding exon was flanked with loxP sites followed by an nls-LacZ. This strain lacks the neo selection cassette by prior crossing with a Cre strain.
Colony maintenance
ReferencesCell Commun. Adhes., 10:365-369 (2003). 14681043
Mech. Dev., 40:41-52 (2016). 26916723

Health Report

Examination Date / Room / Rack2020/10/05Room:3-3Rack:B

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Gjc1gap junction membrane channel protein alpha 711Gjc1<tm2.3Kni>targeted mutation 2.3, Kiyomasa Nishii

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)11

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP11loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP11loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
nlsSV40 large T antigen nuclear localization signal (NLS)11nls

Information2

Donor DNAphage P1 loxP sites, SV40 large T antigen nuclear localization signal (NLS), E. coli beta-galactosidase (lacZ), SV40 polyA signal, mouse Gjc1 genomic DNA
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nishii K et al., Mech Dev. 2016 May;140:41-52.
DepositorKiyomasa Nishii (National Defense Medical College)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-
Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data