Strain Data Sheet

RBRC10867

Strain Information

Image
BRC No.RBRC10867
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameC57BL/6-Chil3<em1Jnam>
Former Common nameECF-L KO
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Jun Namiki at Keio University. C57BL/6J genetic background.
Strain descriptionMutant mice harboring 11 bp deletion in Exon 3 of the Chil3 (chitinase-like 3) gene.
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Chil3
MGI:1330860		chitinase-like 33Chil3<em1Jnam> endonuclease-mediated mutation 1, Jun Namiki

Phenotype

Phenotype annotation from literatures by Mammalian phenotype ontology
Detailed phenotype data

Ordering Information

Donor DNApX330-U6-Chimeric_BB-CBh-hSpCas9[human U6 promoter, S. pyogenes gRNA scaffold, human U6 terminator, CMV,chicken hybrid CMV enhancer/chicken beta-actin promoter (CBh), Synthetic DNA 3xFLAG, SV40 nuclear localization signal(NLS), Streptococcus pyogenes SpCas9 (human codon-optimized), bovine GH polyA signal, AAV2 inverted terminal repeat (ITR), f1 phage f1 origin, E. coli Ampicillin resistance gene (AmpR), E. coli pUC origin], 11 bp deletion of the Chil3 (chitinase-like 3) gene
Research application
Specific Term and ConditionsThe availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
DepositorJun Namiki (Keio University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data