Strain Data Sheet

RBRC10866

Information1

Image
BRC No.RBRC10866
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Hras<tm1.1(G12S)Tumg>
Former Common nameHrasG12S/+ (Hras G12S knock-in mice; C57BL/6J background)
H-2 Haplotype
ES Cell lineBruce 4 [B6.Cg-Thy1<a>]
Background strain
Appearance
Strain developmentDeveloped by Yoko Aoki and Shin-ichi Inoue, Tohoku University School of Medicine in 2018. BRUCE-4 ES cells were used. C57BL/6 congenic line.
Strain descriptionKnock-in mutants in which a point mutation (G12S) was introduced at Exon 1 of the Hras gene. Floxed cDNA/stop cassette and Frt-flnaked Neo cassette were removed by crossing with Cre mice. BALB/C congenic strain is also available as RBRC11578.
Colony maintenance
ReferencesEBioMedicine, 27:138-150 (2018). 29254681

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
HrasHarvey rat sarcoma virus oncogene7Hras<tm1.1(G12S)Tumg>targeted mutation 1.1, Yoko Aoki

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP7loxP

Information2

Donor DNABacteriophage P1 loxP site, mouse Hras genomic DNA
Research applicationApoptosis Research
Cancer Research
Cardiovascular Research
Cre/loxP system
Hematological Research
Metabolism Research
Mouse Models for Human Disease
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. EBioMedicine. 2018 27:138-150In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICALRESOURCE.
DepositorYoko Aoki (Tohoku University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data