Strain Data Sheet

RBRC10853

Strain Information

Image
BRC No.RBRC10853
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6N-Ctsc<tm1Ike>
Former Common nameCatC STOPtetO knockin
H-2 Haplotype
ES Cell lineRENKA [C57BL/6NCrlCrlj]
Background strain
Appearance
Strain developmentDeveloped by Kazuhiro Ikenaka at National Institute for Physiological Sciences in collaboration with Kenji Sakimura at Niigata University. RENKA ES cells derived from C57BL/6N were used to generate this strain. C57BL/6N background. Homozygous mice are viable and fertile.
Strain descriptionKnockin mice in which a STOP-tetO cassette was placed upstream of the translation initiation site of the Ctsc (Cathepsin C) gene.Inserted DNA cassette contains loxP-FRT-Neo-poly A-FRT-tetO-loxP. F
Colony maintenance
ReferencesGlia, 65(6):917-930 (2017). 28251676

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Ctsccathepsin C7Ctsc<tm1Ike>targeted mutation 1, Kazuhiro Ikenaka

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site7Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP7loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)7mouse Phosphoglycerate kinase promoter (PGK promoter), synthetic bacterial EM7 promoter (T7 phage)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA signal7

Ordering Information

Donor DNAマウス PGK プロモーター, synthetic bacterial EM7 promoter (T7 phage),トランスポゾン Tn5由来 Neo 耐性遺伝子, tetracycline operator site 酵母 His3 5’ UTR, FRT SV40 polyA シグナル CMV 最小プロモーター バクテリオファージP1 loxP
Research applicationCre/loxP system
FLP/frt system
Tet system
Specific Term and ConditionsNo specific terms and conditions. (The DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of the BIOLOGICAL RESOURCE.)
DepositorNobuhiko Ohno (National Institute for Physiological Sciences)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data