RBRC10850

Information1

Image
BRC No.RBRC10850
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;B6129-Tet3<tm1.1Hko>
Former Common nameTET3
H-2 Haplotype
ES Cell lineM1 [(C57BL/6 x 129)F1]
Background strain
Appearance
Strain development
Strain descriptionTet3 gene floxed mice. Gene targeting for the Tet3 allele was done by flanking exons 8 and 9 with Cre-loxP sequences. Conditional deletion of Tet3 (exons 8 and 9) can be achieved by crossing the mice with specific cre deleters such as CreERT2. The TET3 protein is a dioxygenase that catalyzes the conversion of 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) and further oxidized cytosine (5fC and 5caC), and plays a key role in active DNA demethylation. Gdf9-Cre-driven Tet3 deletion in early oocytes resulted in a complete absence of Tet3 messenger RNA in Tet3mat-MII oocytes and Tet3 protein in zygotes (Amouroux et al., 2016). This results in a lack of 5hmC formation in the paternal pronucleus following fertilization in zygotes. This conditional allele would be useful to study the roles of Tet3 in DNA de-methylation by combining with tissue specific Cre deleters.
Colony maintenance
References

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Tet3tet methylcytosine dioxygenase 36Tet3<tm1.1Hko>targeted mutation 1.1, Haruhiko Koseki

Gene symbolGene nameChr.Allele symbolAllele namePromoter
frtyeast FRT (flippase recombination target) site6frt

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
loxPphage P1 loxP6loxP

Information2

Donor DNAP1 phage loxP sites, yeast FRT (flipase recombination target) site, mouse Tet3 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
DepositorHaruhiko Koseki (RIKEN)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.

BRC mice in Publications

No Data