RBRC10849

Information1

Image
BRC No.RBRC10849
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;B6129-Tet2<tm1.1Hko>
Former Common nameTET2
H-2 Haplotype
ES Cell lineM1 [(C57BL/6 x 129)F1]
Background strain
Appearance
Strain development
Strain descriptionTet2 gene floxed mice. Gene targeting for the Tet2 allele was done by flanking exon 9 with Cre-loxP sequences. Conditional deletion of Tet2 (exon 9) can be achieved by crossing the mice with specific cre deleters such as CreERT2. The TET2 protein is a dioxygenase that catalyzes the conversion of 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) and further oxidized cytosine (5fC and 5caC), and plays a key role in active DNA demethylation. This conditional allele would be useful to study the roles of Tet2 in DNA de-methylation by combining with tissue specific Cre deleters.
Colony maintenance
References

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Tet2tet methylcytosine dioxygenase 23Tet2<tm1.1Hko>targeted mutation 1.1, Haruhiko Koseki

Information2

Donor DNA酵母 FRT配列 バクテリオファージP1 loxP
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
DepositorHaruhiko Koseki (RIKEN)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.

BRC mice in Publications

No Data