Strain Data Sheet

RBRC10844

Information1

Image
BRC No.RBRC10844
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Rit1<tm1.1(A57G)Tumg>
Former Common nameRit1A57G/+ (Rit1 A57G knock-in mice; C57BL/6J background)
H-2 Haplotype
ES Cell lineBruce 4 [B6.Cg-Thy1<a>]
Background strain
Appearance
Strain developmentDeveloped by Yoko Aoki and Shin-ichi Inoue, Tohoku University School of Medicine in 2019. BRUCE-4 ES cells were used. C57BL/6 congenic line.
Strain descriptionKnock-in mutants in which a point mutation (A57G) was introduced at Exon 4 of the Rit1 gene. Floxed stop cassette and Frt-flnaked Neo cassette at intron 3 were removed by crossing with Cre mice.
Colony maintenance
ReferencesEBioMedicine, 42:43-53 (2019). 30898653

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Rit1Ras-like without CAAX 13Rit1<tm1.1(A57G)Tumg>targeted mutation 1.1, Yoko Aoki

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP3loxP

Information2

Donor DNABacteriophage P1 loxP site, mouse Rit1 genomic DNA
Research applicationCre/loxP system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. EBioMedicine, 42:43-53 (2019).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorYoko Aoki (Tohoku University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data