Strain Data Sheet
RBRC10843
Information1 | |
|---|---|
| Image | |
| BRC No. | RBRC10843 |
| Type | Targeted Mutation Congenic |
| Species | Mus musculus |
| Strain name | B6.Cg-Rit1<tm1(A57G)Tumg> |
| Former Common name | Rit1A57G Neo/+ (C57BL/6J background) |
| H-2 Haplotype | |
| ES Cell line | Bruce 4 [B6.Cg-Thy1<a>] |
| Background strain | |
| Appearance | |
| Strain development | Developed by Yoko Aoki and Shin-ichi Inoue, Tohoku University School of Medicine in 2019. BRUCE-4 ES cells were used. C57BL/6 congenic line. |
| Strain description | Knock-in mutants in which a point mutation (A57G) was introduced at Exon 4 of the Rit1 gene. Floxed stop cassette and Frt-flnaked Neo cassette remain at intron 3. |
| Colony maintenance | |
| References | EBioMedicine, 42:43-53 (2019). 30898653 |
Health Report | |
|---|---|
| Examination Date / Room / Rack | |
Gene | |
|---|---|
| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site3Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site3Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SAAdenovirus SA(splice acceptor site)-stop3SA Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP3loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP3loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene3mouse phosphoglycerate kinase promoter (PGK promoter) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 polyA signal3 |
Information2 | |
|---|---|
| Donor DNA | Bacteriophage P1 loxP sites, Adenovirus SA(splice acceptor site)-stop, Polyomavirus SV40 polyA signal, Saccharomyces cerevisiae FRT sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistant gene, mouse Rit1 genomic DNA |
| Research application | Cre/loxP system FLP/frt system |
| Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. EBioMedicine, 42:43-53 (2019).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. |
| Depositor | Yoko Aoki (Tohoku University) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
| Additional Info. | Genotyping protocol -PCR- Necessary documents for ordering: |
BRC mice in Publications |
|---|
| No Data |