RBRC10817

Information1

Image
BRC No.RBRC10817
TypeCRISPR/Cas9 (Transgene)Cartagena
SpeciesMus musculus
Strain nameSTOCK Prss46<em1Osb>
Former Common namePrss46-11542/-11542
H-2 Haplotype
ES Cell lineEGR-G01 [129S2 x C57BL/6NCr-Tg(CAG/Acr-EGFP)]
Background strain
Appearance
Strain development
Strain description
Colony maintenance
ReferencesBiol. Reprod., 102(1):84-91(2020). 31403672

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Prss46protease, serine 469Prss46<em1Osb>endonuclease-mediated mutation 1, Research Institute for Microbial Diseases, Osaka University

Information2

Donor DNAhuman hU6 promoter CMV enhancer (CBh) chicken chicken beta-actin promoter (CBh) SV40 nuclear localization signal Streptococcus pyogenes SpCas9 (human codon-optimized), crRNA, tracrRNA Escherichia coli ampicillin resistant gene Streptomyces alboniger puromycin resistant gene Mouse a part of Prss46 gene
Research application
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Biol Reprod. 2020 Feb 12;102(1):84-91.The RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorMasahito Ikawa (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Category I MTA: CRISPR/Cas9 genome edited bioresources ( Japanese / English )
Lab HP

BRC mice in Publications

No Data