Strain Data Sheet



BRC No.RBRC10799
SpeciesMus musculus
Strain nameC57BL/6-Tg(Slc32a1-tdTomato)54Rkan
Former Common nameVGAT-tdTomato line #54
H-2 Haplotype
ES Cell line
Background strain
Strain developmentDeveloped by Ryosuke Kaneko and Yuchio Yanagawa, Gunma University in 2010. C57BL/6J genetic background. Transgene is integrated around Gm12442 at Chromosome 4.
Strain descriptionBAC transgenic mice (line 54) expressing tdTomato under the control of the Slc32a1 promoter. Stop cassette between Slc32a1 promoter and tdTomato was removed by crossing with Cre mice.
Colony maintenance
ReferencesJ. Comp. Neurol., 526(3):373-396 (2018). 29063602

Health Report

Examination Date / Room / Rack2022/01/24Room:3-BRack:D


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site4Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
HBB2rabbit beta-globin polyA4HBB2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP4loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
tdTomatotandem dimeric Red Fluorescent Protein (Discosoma sp.)4tdTomato


Donor DNAmouse Slc32a1 promoter, mouse Gad2(GAD65) intron, phage P1 loxP site, yeast FRT (flippase recombination target) site, Discosoma sp.tdTomato cDNA, rabbit beta-globin polyA
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Comp. Neurol., 526(3):373-396 (2018).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results form the use of the BIOLOGICAL RESOURCE.
DepositorRyosuke Kaneko (Gunma University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Genotyping protocol -PCR-
Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: FP license of TBUSA
  3. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data