Strain Data Sheet

RBRC10787

Strain Information

Image
BRC No.RBRC10787
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameC57BL/6N-Vps51<em1Card>
Former Common nameVps51KO
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Michihiko Sugimoto and Kimi Araki at Card, Kumamoto University in 2016. 6NK-7 ES cells derived from C57BL/6N were used to generate this strain. C57BL/6N genetic background.
Strain descriptionVsp51 mutant mice with 35 bp deletion at Exon 1.
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Vps51VPS51 GARP complex subunit19Vps51endonuclease-mediated mutation 1, Center for Animal Resources & Development
  • pontocerebellar hypoplasia, type 13(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
    Detailed phenotype data

    Ordering Information

    Donor DNAAfter creating this strain using the CRISPR/Cas9 system, cas9 was not detected by PCR using Marker Gene Detection kit (TOYOBO, Osaka, Japan). Other introduced genes were not tested.
    Research application
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. 本系統を用いて公表された第一報の論文In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent form the DEPOSITOR.
    DepositorKimi Araki (Kumamoto University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data