Strain Data Sheet

RBRC10784

Information1

Image
BRC No.RBRC10784
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6N-Vps52<tm3.1(EGFP)Card>
Former Common nameVps52-EGFP
H-2 Haplotype
ES Cell line6NK-7 [C57BL/6N]
Background strain
Appearance
Strain developmentDeveloped by Michihiko Sugimoto and Kimi Araki at Card, Kumamoto University in 2018. 6NK-7 ES cells derived from C57BL/6N were used to generate this strain. C57BL/6N genetic background.
Strain descriptionKnockin mice in which EGFP was inserted at the 3’ terminal of Vps52 protein coding sequence to produce Vps52 fusion protein.
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Vps52VPS52 GARP complex subunit17Vps52<tm3.1(EGFP)Card>targeted mutation 3.1, Center for Animal Resources & Development

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)17EGFP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP17loxP

Information2

Donor DNAjellyfish EGFP cDNA, Phage P1 loxP site, mouse Vps52 genomic DNA
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. 本系統を用いて公表された第一報の論文In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent form the DEPOSITOR.
DepositorKimi Araki (Kumamoto University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data