Strain Data Sheet

RBRC10783

Information1

Image
BRC No.RBRC10783
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6N-Cdx2<tm1.1(P2A/mCherry/NLS)Card>
Former Common nameCdx2-mCherry
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Michihiko Sugimoto and Kimi Araki at Card, Kumamoto University in 2018. 6NK-7 ES cells derived from C57BL/6N were used to generate this strain. C57BL/6N genetic background.
Strain descriptionKnockin mice in which P2A-mCherry-SV40NLS was inserted at the 3’ terminal of Cdx2 protein coding sequence. Homozygotes are viable and fertile.
Colony maintenance
References本系統を用いて公表された第一報の論文

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Cdx2caudal type homeobox 25Cdx2<tm1.1(P2A/mCherry/NLS)Card>targeted mutation 1.1, Center for Animal Resources & Development

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
P2Aporcine teschovirus-1 2A peptide5P2A

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP5loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
mCherryRed Fluorescent Protein (Discosoma sp.)5mCherry

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
nlsSV40 large T antigen nuclear localization signal (NLS)5nls

Information2

Donor DNAP2A (porcine teschovirus-1 2A peptide), カイメン mCherry, SV40 SV40核移行シグナル, P1ファージ loxP
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. 本系統を用いて公表された第一報の論文In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent form the DEPOSITOR.
DepositorKimi Araki (Kumamoto University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data