Strain Data Sheet



BRC No.RBRC10771
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.B6CB-Rr45<tm1.1Tshir> Rr(MRCS1)<tm1.1Tshir>/Ms
Former Common nameMRCS1-MFCS4 double knockout (DKO), delta MRCS1; delta MFCS4, MRCS1-/-; MFCS4-/-
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Strain developmentDeveloped by Toshihiko Shiroishi and Tomoko Sagai, National Institute of Genetics in 2013 using TT2 ES cells. C57BL/6 congenic line.
Strain descriptionDeletion mutant of MRCS1 and MFCS4 enhancer of Shh gene.
Colony maintenance
ReferencesSci. Rep., 7(1):13004 (2017). 29021530
Development, 136(10):1665-74 (2009). 19369396

Health Report

Examination Date / Room / Rack


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
9530036O11RikRIKEN cDNA 9530036O11Rik59530036O11Rik<tm1.1Tshir>targeted mutation 1.1, Toshihiko Shiroishi

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Rr45regulatory region 455Rr45<tm1.1Tshir>targeted mutation 1.1, Toshihiko Shiroishi


Donor DNAmouse PGK promoter, E.coli Neomycin resistant gene, bovine poly A signal, P1 phage loxP
Research applicationCre/loxP system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Sci Rep. (2017) 7:13004. Development. (2009)136:1665-74.
DepositorToshihiko Shiroishi (National Institute of Genetics)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data