Strain Data Sheet

RBRC10764

Information1

Image
BRC No.RBRC10764
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Shh<tm1Tshir>/Ms
Former Common nameShhd2EGFP, Shh-d2EGFP KI
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Toshihiko Shiroishi and Takanori Amano, National Institute of Genetics in 2006 using ES cells derived from C57BL/6. C57BL/6 genetic background.
Strain descriptionDegraded form of EGFP was knocked in at the Shh locus.
Colony maintenance
ReferencesDev. Cell. l6(1):47-57 (2009). 19097946

Health Report

Examination Date / Room / Rack2021/03/08Room:2-検Rack:C
2020/12/07Room:2-検Rack:C

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Shhsonic hedgehog5Shh<tm1Tshir>targeted mutation 1, Toshihiko Shiroishi

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)5EGFP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)5mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA signal5

Information2

Donor DNAJellyfish (A. victoria) EGFP cDNA, Mouse The degradation domain of ornithine decarboxylase cDNA, SV40 polyA signal, mouse PGK promoter, E. coli Neomycin resistance gene, mouse Shh genomic DNA
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Amano et al., Dev Cell. 2009 Jan;l6(1):47-57.
DepositorToshihiko Shiroishi (National Institute of Genetics)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)
  4. GFP Transfer License (Japanese / English)
    Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

Genotyping protocol -PCR-

BRC mice in Publications

No Data