RBRC10764

Information1

Image
BRC No.RBRC10764
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Shh<tm1Tshir>/Ms
Former Common nameShhd2EGFP, Shh-d2EGFP KI
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain development
Strain description
Colony maintenance
ReferencesDev. Cell. l6(1):47-57 (2009). 19097946

Health Report

Examination Date / Room / Rack2020/06/08Room:2-検Rack:C

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Shhsonic hedgehog5Shh<tm1Tshir>targeted mutation 1, Toshihiko Shiroishi

Gene symbolGene nameChr.Allele symbolAllele namePromoter
EGFPEnhanced Green Fluorescent Protein, F64L and S65T (Aequorea victoria)5EGFP

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)5mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
SV40 polyA signal5

Information2

Donor DNAJellyfish (A. victoria) EGFP cDNA, Mouse The degradation domain of ornithine decarboxylase cDNA, SV40 polyA signal, mouse PGK promoter, E. coli Neomycin resistance gene, mouse Shh genomic DNA
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Amano et al., Dev Cell. 2009 Jan;l6(1):47-57.
DepositorToshihiko Shiroishi (National Institute of Genetics)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Cryopreserved sperm (within 1 month)
Additional Info.GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.
Genotyping protocol -PCR-

BRC mice in Publications

No Data