Strain Data Sheet



BRC No.RBRC10761
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.B6CB-Gsdmd<tm1.1Tshir>/Ms
Former Common nameGsdmd-/-, Gsdmd KO
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Strain developmentDeveloped by Toshihiko Shiroishi and Tomoaki Fujii, National Institute of Genetics in 2005. TT2 ES cells derived from (C57BL/6 x CBA)F1 were used. Mice were backcrossed to C57BL/6J for more than 10 generations. C57BL/6 congenic line. LacZ reporter was deleted by crossing with Cre mice.
Strain descriptionKnockout mice for Gsdmd.
Colony maintenance
ReferencesGenesis, 46, 418-423 (2008). 18693275

Health Report

Examination Date / Room / Rack2022/08/29Room:4-BRack:I


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Gsdmdgasdermin D15Gsdmd<tm1.1Tshir>targeted mutation 1.1, Toshihiko Shiroishi

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP15loxP


Donor DNAP1 bacteriophage loxP site, mouse Gsdmd genomic DNA
Research applicationCre/loxP system
Immunology and Inflammation Research
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genesis, 46, 418-423 (2008).The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. (Contact info: Tsuyoshi Koide, Ph.D. <>, Mouse Genomics Resource Laboratory)
DepositorToshihiko Shiroishi (National Institute of Genetics)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Live mouse (3 to 6 months)
Additional Info.Mouse of the Month Mar 2020
Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data