Strain Data Sheet

RBRC10757

Strain Information

Image
BRC No.RBRC10757
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129-Mab21l2<tm1Nao>
Former Common nameC57BL/6(Mab21l2tm1Nao), Mab21l2tm1Nao
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearance
Strain developmentlethality throughout fetal growth and development, complete penetranceabnormal heart position or orientationabnormal retina morphologyabnormal ventral body wall morphology
Strain descriptionThis gene is similar to the MAB-21 cell fate-determining gene found in C. elegans. It is required for several aspects of embryonic development including normal development of the eye, heart and body wall. Mutation details: The entire gene coding region was replaced with a neomycin resistance cassette via homologous recombination. Absence of gene expression in homozygous mutants was confirmed by whole mount in situ hybridization analysis of E10.5 homozygous mutant embryos.
Colony maintenance
References
Requirement for Mab21l2 during development of murine retina and ventral body wall.
Yamada R, Mizutani-Koseki Y, Koseki H, Takahashi N
Dev. Biol., 274(2):295-307 (2004). 15385160

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
SV40 polyA3
  • colobomatous microphthalmia-rhizomelic dysplasia syndrome(MedGEN)
    • Mab21l2mab-21-like 23Mab21l2targeted mutation 1, Naoki Takahashi
  • colobomatous microphthalmia-rhizomelic dysplasia syndrome(MedGEN)
    • neoneomycin resistance gene (E. coli)3herpes simplex virus thymidine kinase promoter (HSV tk promoter)
  • colobomatous microphthalmia-rhizomelic dysplasia syndrome(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
    Detailed phenotype data

    Ordering Information

    Donor DNAherpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli Neomycin resistant gene, SV40 PolyA signal, mouse Mab21l2 genomic DNA
    Research application
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
    DepositorNaoki Takahashi (The University of Tokyo)
    Strain Statusan icon for Live miceLive mice
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovery and QC required prior to distribution
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data