RBRC10757

Information1

Image
BRC No.RBRC10757
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129-Mab21l2<tm1Nao>
Former Common nameC57BL/6(Mab21l2tm1Nao), Mab21l2tm1Nao
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearance
Strain developmentlethality throughout fetal growth and development, complete penetranceabnormal heart position or orientationabnormal retina morphologyabnormal ventral body wall morphology
Strain descriptionThis gene is similar to the MAB-21 cell fate-determining gene found in C. elegans. It is required for several aspects of embryonic development including normal development of the eye, heart and body wall. Mutation details: The entire gene coding region was replaced with a neomycin resistance cassette via homologous recombination. Absence of gene expression in homozygous mutants was confirmed by whole mount in situ hybridization analysis of E10.5 homozygous mutant embryos.
Colony maintenance
ReferencesDev. Biol., 274(2):295-307 (2004). 15385160

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Mab21l2mab-21-like 23Mab21l2<tm1Nao>targeted mutation 1, Naoki Takahashi

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)3herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
SV40 polyA3

Information2

Donor DNAherpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli Neomycin resistant gene, SV40 PolyA signal, mouse Mab21l2 genomic DNA
Research application
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
DepositorNaoki Takahashi (The University of Tokyo)
Strain Statusan icon for Live miceLive mice
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Cryopreserved sperm (within 1 month)
Additional Info.

BRC mice in Publications

No Data