Strain Data Sheet

RBRC10756

Strain Information

Image
BRC No.RBRC10756
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129-Mab21l1<tm1Nao>
Former Common nameC57BL/6(Mab21l1tm1Nao), Mab21l1tm1Nao
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearance
Strain developmentR1 ES cells were established in August 1991 from a male blastocyst hybrid of two 129 substrains (129X1/SvJ and 129S1/SV-+p+Tyr-c Kitl Sl-J/+).abnormal male preputial gland morphology abnormal eye morphology abnormal skull development
Strain descriptionThis gene is similar to the MAB-21 cell fate-determining gene found in C. elegans. It may be involved in eye and cerebellum development.Mutation details: The entire gene coding region was replaced with a neomycin resistance cassette via homologous recombination. Absence of gene expression in homozygous mutants was confirmed by whole mount in situ hybridization analysis of E10.5 homozygous mutant embryos.
Colony maintenance
References
Cell-autonomous involvement of Mab21l1 is essential for lens placode development.
Yamada R, Mizutani-Koseki Y, Hasegawa T, Osumi N, Koseki H, Takahashi N
Development, 130(9), 1759-1770 (2003). 12642482

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
SV40 polyA3
  • cerebellar, ocular, craniofacial, and genital syndrome(MedGEN)
    • Mab21l1mab-21-like 13Mab21l1targeted mutation 1, Naoki Takahashi
  • cerebellar, ocular, craniofacial, and genital syndrome(MedGEN)
    • neoneomycin resistance gene (E. coli)3herpes simplex virus thymidine kinase promoter (HSV tk promoter)
  • cerebellar, ocular, craniofacial, and genital syndrome(MedGEN)

    • Phenotype

    Annotation by Mammalian phenotyhpe ontology
    Detailed phenotype data

    Ordering Information

    Donor DNA大腸菌(クラス1) Neomycin resistant gene, 単純ヘルペスウイルス１型(クラス2) Thymidine Kinase promoter, シミアンウイルス40(SV40)(クラス2) PolyA signal
    Research application
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
    DepositorNaoki Takahashi (The University of Tokyo)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data