RBRC10756

Information1

Image
BRC No.RBRC10756
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129-Mab21l1<tm1Nao>
Former Common nameC57BL/6(Mab21l1tm1Nao), Mab21l1tm1Nao
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearance
Strain developmentR1 ES cells were established in August 1991 from a male blastocyst hybrid of two 129 substrains (129X1/SvJ and 129S1/SV-+p+Tyr-c Kitl Sl-J/+).abnormal male preputial gland morphology abnormal eye morphology abnormal skull development
Strain descriptionThis gene is similar to the MAB-21 cell fate-determining gene found in C. elegans. It may be involved in eye and cerebellum development.Mutation details: The entire gene coding region was replaced with a neomycin resistance cassette via homologous recombination. Absence of gene expression in homozygous mutants was confirmed by whole mount in situ hybridization analysis of E10.5 homozygous mutant embryos.
Colony maintenance
ReferencesDevelopment, 130(9), 1759-1770 (2003). 12642482

Health Report

Examination Date / Room / RackNo Data

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele namePromoter
Mab21l1mab-21-like 13Mab21l1<tm1Nao>targeted mutation 1, Naoki Takahashi

Gene symbolGene nameChr.Allele symbolAllele namePromoter
neoneomycin resistance gene (E. coli)3herpes simplex virus thymidine kinase promoter (HSV tk promoter)

Gene symbolGene nameChr.Allele symbolAllele namePromoter
SV40 polyA3

Information2

Donor DNA大腸菌(クラス1) Neomycin resistant gene, 単純ヘルペスウイルス1型(クラス2) Thymidine Kinase Promotor, シミアンウイルス40(SV40)(クラス2) PolyA signal
Research application
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
DepositorNaoki Takahashi (The University of Tokyo)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.

BRC mice in Publications

No Data