Strain Data Sheet
RBRC10754
Strain Information | |
|---|---|
| Image | |
| BRC No. | RBRC10754 |
| Type | Transgene |
| Species | Mus musculus |
| Strain name | C57BL/6J-Tg(Rspo2-cre)BIto |
| Former Common name | Rspo2-B-Cre |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | |
| Appearance | |
| Strain development | Deposited by Shigeyoshi Itohara, RIKEN Center for Brain Science. A BAC DNA construct was injected to fertilized eggs derived from C57BL/6J. Mice were maintained by crossing to C57BL/6J. C57BL/6 genetic background. |
| Strain description | Transgenic mice (line B) expressing Cre under the control of Pspo2 promoter. |
| Colony maintenance | |
| References | Antagonistic negative and positive neurons of the basolateral amygdala. Kim J, Pignatelli M, Xu S, Itohara S, Tonegawa S Nat. Neurosci., 1636-1646 (2016). 27749826 |
Health Report | |
|---|---|
| Examination Date / Room / Rack | 2025/09/01Room:3-ARack:DSentinel mouse program 2024/04/08Room:3-ARack:DSentinel mouse program |
Gene | |||||||
|---|---|---|---|---|---|---|---|
| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| ACTB | human beta-actin polyA | UN | ACTB | ||||
| Frt | yeast FRT (flippase recombination target) site | UN | Frt | ||||
| cre | Phage P1 Cre recombinase | UN | cre | ||||
| nls | SV40 large T antigen nuclear localization signal (NLS) | UN | nls | ||||
Phenotype | |
|---|---|
| Phenotype annotation from literatures by Mammalian phenotype ontology | |
| Detailed phenotype data | |
Ordering Information | |
|---|---|
| Donor DNA | mouse Rspo2 genomic DNA, SV40 Nuclear Localization Signal(NLS), P1 phage Cre, human beta-actin transcriptional regulatory region, yeast FRT (flippase recombination target) site (data update: May 30, 2023) |
| Research application | Cre/loxP system FLP/frt system Cre driver |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat. Neurosci., 1636-1646 (2016). |
| Depositor | Shigeyoshi Itohara (RIKEN) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
|---|
Lim H, Zhang Y, Peters C, Straub T, Mayer JL, Klein R. Genetically- and spatially-defined basolateral amygdala neurons control food consumption and social interaction. Nat Commun 15(1) 6868(2024) 39127719 |