Strain Data Sheet

RBRC10745

Strain Information

Image
BRC No.RBRC10745
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameC57BL/6J-Tg(tetO-YC2.60)#Ito
Former Common nameTRE-YC2.60
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Shigeyoshi Itohara at RIKEN.
Strain descriptionTrasgenic mice expressing YC2.60 under the control of teto promoter.
Colony maintenance
References

Health Report

Examination Date / Room / Rack2024/04/08Room:3-BRack:B
2024/01/09Room:3-BRack:B
2023/10/10Room:3-BRack:B
2023/07/10Room:3-BRack:B

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
SV40 Intron polyAUN
WPREWoodchuck hepatitis virus posy-transcriptional regulatory element (WPRE)UNWPRE
YC2.60Yellow Cameleon 2.60 (Aequorea victoria ECFP cDNA, Xenopus laevis calm2 cDNA, calmodulin (CaM)-binding peptide (rabbit skeletal muscle myosin light chain kinase (MLCK) cDNA), circularly permuted form of the Venus yellow fluorescent protein cDNA)UNYC2.60E. coli tetO (tetracycline operator)

Phenotype

Annotation by Mammalian phenotyhpe ontology
Detailed phenotype data

Ordering Information

Donor DNAE. coli tetO (tetracycline operator), Yellow Cameleon 2.60 (Aequorea victoria ECFP cDNA, Xenopus laevis calm2 cDNA, calmodulin (CaM)-binding peptide (rabbit skeletal muscle myosin light chain kinase (MLCK) cDNA), circularly permuted form of the Venus yellow fluorescent protein cDNA), Woodchuck hepatitis virus posttranscriptional regulatory element (WPRE), SV40 Intron polyA
Research applicationFluorescent Proteins/lacZ System
Tet system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE containing Venus, an acknowledgment to Dr. Atsushi Miyawaki and a citation of the following literature(s) designated by the DEPOSITOR are requested.
Nat. Biotechnol., 20, 87-90 (2002).
DepositorShigeyoshi Itohara (RIKEN)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-
Sequence of Y2.60

BRC mice in Publications

No Data